Fig. 2: InR knock-down in the fat body modulates regeneration and lipid storage.

a Expression of insulin-like peptides (Dilps) measured by qPCR. The expression in regenerating (3%DSS, circles) animals of Dilp2, Dilp3 and Dilp5 is shown as fold change to control (dots). (n = 5, each sample = 5 animals pooled, unpaired t-test, *p < 0.05). Mean and SD are indicated. b TAG yields normalized to PE and the average corresponding control signal in fat body samples of WT (black), FB-InR^KD (lpp > InR^RNAi, brown) and FB-PI3K^KD (lpp > PI3K^RNAi, purple) flies during control conditions (dots) and regeneration (3% DSS, circles). -Welch’s t-test, *p < 0.05). n = 15 (WT control), 18 (WT 3% DSS), 12 (InR^KD, control and 3% DSS), 6 (PI3K^KD control) and 10 (PI3^KD 3% DSS), 3 animals pooled per sample. Mean and SD are indicated. c Sterol yields normalized to PE in fat body samples. Sample description, see (b). n = 15 (WT control), 18 (WT 3%DSS), 12 (InR^KD, control and 3% DSS), 6 (PI3K^KD control) and 10 (PI3^KD 3% DSS), 3 animals pooled per sample. Mean and SD are indicated. d Sterol yields normalized to PE in midgut samples. Sample description, see (ba)., Welch’s t-test **p < 0.01. n = 16 (WT control), 15 (WT 3% DSS), 17 (InR^KD, control), 14 (InR^KD 3% DSS), 12 (PI3K^KD control and 11 (PI3^KD 3% DSS). 2 animals were pooled per sample. Mean and SD are indicated. e Quantification of pH3 positive cells per midgut in WT and InR^KD flies in control condition and upon regeneration. Welch’s t-test, **p < 0.001, *p < 0.05. Wild type: n = 7 (Control), n = 8 (3% DSS); InR^KD: n = 6 (both conditions). Mean and SD are indicated. f Graphic overview depicting the InR knock-down strategy in the fat body cells. DSS treatment in the InR^KD results in markedly reduced intestinal stem cell division rates in regenerating animals.