Fig. 4: Glial scarring, neural stem cell, and neurogenesis post-stroke responses.
From: Stroke-induced neuroplasticity in spiny mice in the absence of tissue regeneration
Immunofluorescence was performed on 30 µm thick brain slices. a Brain sections at 2 dpi stained for SOX2 (top, magenta), GFAP (second, green), DCX (third, yellow), and merged with DAPI in blue (bottom). Two representative brain regions with the left depicting the SVZ, striatum and cortex and the right hippocampus, midbrain, and cortex. The ipsilateral hemisphere is the right hemisphere and the contralateral is the left, and boxed areas denote regions used for enlargement. Enlarged images of areas of glial scarring and neurogenesis are provided showing SVZ/striatum (b) and hippocampus (c) at 2 dpi. d Same description as (a), except for 175 dpi. Enlarged images of areas with glial scarring and neurogenesis are provided showing SVZ/striatum (e) and hippocampus (f). g Quantification of the area of activated astrocytes (astrogliosis) as a percentage of the area of the brain hemisphere. h Quantification of the number of GFAP/SOX2 co-positive cells per 100 µM of the dentate gyrus. i Quantification of the number of DCX positive stains per 100 µM of the dentate gyrus. All quantification shown at four timepoints from uninjured (baseline) to 2, 30, and 175 dpi where blue circles denote the contralateral hemisphere and red squares denote the ipsilateral hemisphere. Whole brain scale bars = 1 mm, Zoom scale bars = 100 µm. 2way ANOVA performed with Tukey correction for multiple comparisons (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).
