Fig. 4: RRAD overexpression in human heart slices promotes cell cycle induction and induces more CMs to enter the cell cycle in response to cell cycle stimuli. | npj Regenerative Medicine

Fig. 4: RRAD overexpression in human heart slices promotes cell cycle induction and induces more CMs to enter the cell cycle in response to cell cycle stimuli.

From: Pharmacological or genetic inhibition of LTCC promotes cardiomyocyte proliferation through inhibition of calcineurin activity

Fig. 4

a Representative images of human heart slices transfected with LacZ or RRAD adenovirus for 24 h, then transfected with CDK4 and CCND (2 F) for 48 h and stained against troponin-T (green), PHH3 (red) and Dapi (blue) (Scale bar 20 µm). b–c Quantification of the percentage PHH3 positive CM nuclei (n = 4–6 individual slices). d Representative images of human heart slices transduced with LacZ or RRAD adenovirus for 24 h, then transduced with CDK4 and CCND (2 F) for 48 h and stained against troponin-T (green), Aurora Kinase B (red), and Dapi (blue) (Scale bar 20 µm). e, f Quantification of the percentage of Aurora Kinase B positive CM nuclei (n = 8). Data are presented as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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