Fig. 3: Cigarette smoke reduces fibroblast-derived osteoglycin expression in the lungs.
From: Fibroblast-derived osteoglycin promotes epithelial cell repair
a ScRNA-Seq analysis of Ogn expression by cell type in murine lungs. b ScRNA-Seq analysis showing cell type specificity of OGN in the human lung, with red arrow indicating OGN in alveolar fibroblasts. c Image of OGN staining in lung tissue of never-smoker (scale = 500 µm), with close-up showing an artery and an airway. d Ogn gene expression in PCLS treated with 15% CSE (mean ± SEM, N = 5, paired t-test on log-transformed ΔCt values). e Pseudobulk ScRNA-Seq of Ogn in fibroblasts from lung tissue of mice exposed to two, four, and six months of CS compared to filtered air (FA). f Normalized counts from DESeq2 analysis of Ogn in fibroblasts upon treatment with a COPD-related exacerbation cocktail (mean ± SEM, N = 3-4). g, h OGN staining images in lung tissue of current smoker and ex-smoker donor (scale = 500 µm). i Percentage (%) of positively stained area for OGN in whole lung tissue and parenchyma in never, current, and ex-smokers. j Intensity of positive staining for OGN in whole lung tissue and parenchyma of never, current, and ex-smokers. Statistically significant comparisons: *p < 0.05, **p < 0.01.
