Table 1 RT-qPCR primers to validate BMP expression and evaluate osteogenic gene expression and differentiation
Gene | Species | Direction | Sequence |
|---|---|---|---|
BMP2 (codon-optimized) | Human | Forward | CCTCTGCACAAGAGAGAGAAGA |
Reverse | CACATCGCTGAAGTCCACATAC | ||
BMP7 (codon-optimized) | Human | Forward | GCCGACATGGTGATGAGTT |
Reverse | CGGAATTCTCTGTGGTGGTATC | ||
GAPDH | Human | Forward | GTCTCCTCTGACTTCAACAGCG |
Reverse | ACCACCCTGTTGCTGTAGCCAA | ||
OCN | Mice | Forward | GGAGGGCAATAAGGTAGTGAAC |
Reverse | CAAGCCATACTGGTCTGATAGC | ||
OPN | Mice | Forward | AGACCGTCACTGCTAGTACA |
Reverse | CAGTCCATAAGCCAAGCTATCA | ||
SP7 | Mice | Forward | CACCCTTCCCTCACTCATTT |
Reverse | CCTTGTACCACGAGCCATAG | ||
RUNX2 | Mice | Forward | ACCACTCACTACCACACGTA |
Reverse | GGATGCTGACGAAGTACCATAG | ||
GAPDH | Mice | Forward | CATCACTGCCACCCAGAAGACTG |
Reverse | ATGCCAGTGAGCTTCCCGTTCAG |
