Fig. 2: Treatment with NF-kB inhibitors results in an abnormal inflammatory gene expression profile in the regenerating fin. | npj Regenerative Medicine

Fig. 2: Treatment with NF-kB inhibitors results in an abnormal inflammatory gene expression profile in the regenerating fin.

From: An NF-kB/TNF-alpha signalling feedback loop acts to coordinate tissue regeneration and macrophage behaviour in zebrafish

Fig. 2

Uninjured or tail amputated 3 dpf larvae were treated with DMSO (control), 2.5 mM BMS-345541 or 300 µM JSH-23 until 3 hpa (A–C) or 6 hpa (D–F). Extracted RNA from pooled animals (n = 60 animals/condition) was used as template for RT-qPCR and fold difference plotted for injured relative to uninjured animals for each condition. Gene expression of ikbaa (G, H), ikbab (I, J), il1b (K, L) and tnfa (M, N) was examined by in situ hybridisation in control (G, I, K, M) and BMS-354451 treated (H, J, L, N) larvae at 3 hpa; arrows indicate expression at the wound site. Significance for relative gene expression difference between conditions was calculated using the REST software after correcting for primer efficiency (A–F). Significance for differences between groups is calculated using a randomisation test in REST, which compares across conditions (***p < 0.001, **p < 0.01, *p < 0.05, n.s. not significant). Bar plots show mean values with bars representing the standard error of the mean. Scale bars 100 µm (G–N).

Back to article page