Fig. 2: LncBAR deficiency inhibits cardiomyocyte proliferation in postnatal hearts. | npj Regenerative Medicine

Fig. 2: LncBAR deficiency inhibits cardiomyocyte proliferation in postnatal hearts.

From: Long noncoding RNA LncBAR enhances BRG1 protein to promote cardiomyocyte cell cycle progression and cardiac repair

Fig. 2: LncBAR deficiency inhibits cardiomyocyte proliferation in postnatal hearts.The alternative text for this image may have been generated using AI.

a Schematic depiction of LncBAR knockout (KO) mice and the genotyping strategy. F and R, primers designed for genotyping. b Representative genotyping results from wild-type and LncBAR KO (LncBAR−/−) mice using PCR primer pairs shown in (a) (n = 3 mice). c Expression level of LncBAR in WT and LncBAR−/− hearts as evaluated by RT-qPCR (n = 3 mice). Representative confocal z-stack images and quantification of cTnT+ Ki67+ (d) or cTnT+ pH3+ CMs (e) in heart sections from neonatal WT and LncBAR−/− hearts at P3. Nuclei were labeled with DAPI. White arrows indicate Ki67+ or pH3+ CMs (n = 15 fields, 3 mice/group). Scale bars, 20 µm f, g Representative confocal z-stack images and quantification of cTnT+ Ki67+ (f) or cTnT+ pH3+ CMs (g) in heart sections from neonatal WT and LncBAR−/− hearts at P7. Nuclei were labeled with DAPI. White arrows indicate Ki67+ or pH3+ CMs (n = 15 fields, 3 mice/group). Scale bars, 20 µm. Data are presented as mean ± SEM. Statistical significance was determined by two-tailed unpaired t test. **P < 0.01, ***P < 0.001.

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