Fig. 3: LncBAR depletion inhibits neonatal heart regeneration. | npj Regenerative Medicine

Fig. 3: LncBAR depletion inhibits neonatal heart regeneration.

From: Long noncoding RNA LncBAR enhances BRG1 protein to promote cardiomyocyte cell cycle progression and cardiac repair

Fig. 3: LncBAR depletion inhibits neonatal heart regeneration.The alternative text for this image may have been generated using AI.

a Schematic depiction of the experimental design in WT and LncBAR−/− neonatal mice. b, c Representative confocal z-stack images and quantification of Ki67+ cTnT+ CMs (b), and pH3+ cTnT+ CMs (c) from WT and LncBAR−/− heart sections in the apex zone at day 7 after AR (AR7). White arrows indicate Ki67+ or pH3+ CMs (n = 15 fields, 3 mice/group). Scale bars, 20 µm. Masson’s trichrome staining and quantification of fibrotic area (blue) in sagittal heart sections from WT and LncBAR−/− mice at AR7(d) and AR21(e) (n = 5 mice). Scale bars, 1 mm. Echocardiography showing left ventricular ejection fraction (f) and fractional shortening (g) at AR21 of WT and LncBAR−/− mice (n = 5 mice for sham groups, n = 8 mice for AR groups). Data are presented as mean ± SEM. Statistical significance was determined by two-tailed unpaired t test or two-way ANOVA with Tukey’s multiple comparisons test. **P < 0.01, ***P < 0.001.

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