Fig. 3: Quantification of culture activation.
From: Label-free in-line characterization of immune cell culture using quantitative phase imaging
A–F qOBM phase images of cell cultures from naive D0 cells to activated D3-10 cell cultures, a partially activated culture on D14, and a post-activation D21 culture. G Confusion matrix of activated and quiescent cell cultures classification using qOBM phase images. H Scatter plot of the qOBM predicted culture activation vs flow cytometry measurements tested on cultures from 11 unique donors. I, J qOBM phase image and phase and fluorescence overlay. Blue cells are quiescent and the red cells are activated. K Clustergram showing the correlation of curated image features of activated and quiescent cells. L UMAP of all tested activated, post-activation, or naive cells which shows distinct clusters of the three groups. Representative cell images from each cluster are also included. This UMAP was created based on the features seen in (K). M Percent stacked barchart of the percentage of cells in each group by day of culture (solid) compared to those measured with flow cytometry (striped). Scale bars are 100 μm.
