Fig. 4: Characterization of T cell cultures in-line.
From: Label-free in-line characterization of immune cell culture using quantitative phase imaging
A Flow chart depicting the process in-line T cells characterization with qOMB. First, cells are segmented, then the viability residual network is applied to determine culture viability, and finally, the activation network is applied to the live cells to determine the culture activation. B Photograph of the qOBM system integrated into the incubator. C Scatter plot of the in-line qOBM measured viability vs flow cytometry. D Scatter plot of the in-line qOBM measured activation vs flow cytometry. E–G Representative images of classified T cells acquired in-line. Cells highlighted in red are dead, in blue are quiescent, and in green are activated. Scale bars are 100 μm.
