Fig. 5: miR-140-engineered ASC-Exos demonstrate enhanced therapeutic efficacy in both subacute and chronic OA rat models. | npj Regenerative Medicine

Fig. 5: miR-140-engineered ASC-Exos demonstrate enhanced therapeutic efficacy in both subacute and chronic OA rat models.

From: Deer antler ASCs exosomes ameliorate osteoarthritis via miR-140/MMP13 axis-mediated dual modulation of inflammation and cartilage regeneration

Fig. 5: miR-140-engineered ASC-Exos demonstrate enhanced therapeutic efficacy in both subacute and chronic OA rat models.

A Schematic illustration of the experimental design showing the establishment of subacute and chronic OA models and subsequent treatment regimens with engineered exosomes. Created in BioRender. Yuhao, S. (2025) https://BioRender.com/6vmrokj. B Macroscopic appearance of articular cartilage from subacute (upper panel) and chronic (lower panel) OA models following different treatments. C Histological analysis of cartilage tissues in the subacute OA model. H&E staining (upper panel) and S-F staining (lower panel) showing cartilage structure and proteoglycan content, respectively. Quantitative assessment using the OARSI scoring system is presented in the corresponding bar graphs (right panel). Scale bars: 500 μm. D Histological evaluation of cartilage tissues in the chronic OA model. H&E staining (upper panel) and S-F staining (lower panel) reveal more severe cartilage degradation. Quantitative analysis using the OARSI scores is shown in the corresponding bar graphs (right panel). Scale bars: 500 μm. E Immunohistochemical analysis of COL II, NLRP3, and MMP13 in the subacute OA model. Scale bars: 500 μm. F Immunohistochemical analysis of COL II, NLRP3, and MMP13 in the chronic OA model. Scale bars: 500 μm.

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