Fig. 6: IgG subclasses separation and DNA hydrolysis.
From: DNA hydrolysing IgG catalytic antibodies: an emerging link between psychoses and autoimmunity
a About 50 µg of pre-purified total IgG Abs (twofold dilution) suspended in 25 mM Tris-HCl pH 7.4 was fractionated and eluted using 25 mM Tris-HCl pH 7.4 + 0.2 M NaCl. Peak I-flow-through, Peak II-elution. Inset: IgG subclasses in the peak I and II were discriminated by separate western blots. b DNA hydrolysis exhibited by peaks I (lane I) and II (lane II). UC- scDNA incubated alone; sc supercoiled DNA, rc relaxed circular DNA, ln linear DNA.
