Fig. 1: Identification of ANX A1 as a sesamin metabolite-binding protein using affinity nanobeads.
From: Annexin A1 accounts for an anti-inflammatory binding target of sesamin metabolites
a Scheme of sesamin metabolism in the liver. Sesamin is catalysed into SC1 and subsequently SC2 by cytochromes P450. SC1 or SC2 is further methylated into SC1m or SC2m by catechol O-methyl transferase (COMT). b Scheme of sesamin derivative-conjugation with affinity nanobeads. The carboxylated derivatives are conjugated with amino-modified nanobeads (upper panel). Lower panels show the chemical structures of the beads conjugating with sesamin, SC1, SC1m, or curcumin. c Affinity purification of the SC1-binding protein from U937 cell lysate. The affinity nanobeads conjugating with the ligands were incubated with U937 cell lysate, and bound proteins were analysed with SDS-PAGE before visualising using silver staining. The indicated protein (arrow) was identified as ANX A1 using peptide sequencing via ESI-MS. d Recombinant ANX A1 protein was incubated with affinity nanobeads, and bound proteins were analysed using SDS-PAGE before visualising with silver staining.
