Fig. 3: SC1 suppresses TNFα production dependent on ANX A1 in U937 cells.

a Suppressive effect of sesamin derivatives on TNFα production in U937 cells. U937 cells were differentiated by adding PMA for 24 h and treating with or without 1 µg mL⁻¹ LPS and/or the indicated amount of sesamin, SC1 or SC2 for 12 h. TNFα production was analysed using ELISA. Right panel shows the relative inhibitory percentage of TNFα production by sesamin derivatives. b Protein expression of ANX A1 knockdown (KD) U937 cells. Control and shRNA for ANX A1 introducing lentivirus were infected into U937 cells. The cell lysates were analysed using western blotting using antibody against ANX A1 or β-actin. c U937 cells (control or ANX A1 KD) were differentiated with PMA and treated with or without 1 µg mL⁻¹ LPS and/or the indicated amount of sesamin, SC1 or SC2. TNFα production was analysed using ELISA. All data represent the mean ± SD (n = 3). *p < 0.05, **p < 0.01 using unpaired Student’s t test.