Fig. 6: ANX A1 is required for the suppressive effects of sesamin against CCl4-induced liver inflammation.
From: Annexin A1 accounts for an anti-inflammatory binding target of sesamin metabolites
Effect of sesamin on CCl4–induced inflammation induced in WT or ANX A1-KO mice. WT or ANX A1-KO mice were intraperitoneally injected with CCl4. Sesamin (25 mg kg−1 body weight) or vehicle was administered twice at 1 h before and 7 h after CCl4 administration. The plasma or liver were collected for analysis 12 h after CCl4 administration. Production of TNFα and MCP-1 protein in the plasma (a) or in the liver (b) was analysed using ELISA. c Expression of TNFα, MCP-1, and galectin-3 mRNA in the liver was measured using qPCR, and normalised to 18S ribosomal RNA expression. The graph showed as the relative fold change compared to mRNA expression in the liver treated with vehicle. Data represent the mean ± SE. n = 5-10 mice in each group. *p < 0.05 or **p < 0.01 using unpaired Student’s t test. d The liver section collected 24 h after CCl4 administration with or without sesamin treatment was stained using galectin-3 antibody. Scale bar: 50 μm. e mRNA expression of αSMA or Col1a1 was measured by qPCR from the liver tissues collected 24 h after CCl4 administration with or without treatment of sesamin, and normalised by the expression of 18S rRNA. Data represent the mean ± SE. n = 13–15 mice in each group. *p < 0.05 using unpaired Student’s t test.
