Table 1 Concentrations of total linoleic acid (LA)-derived and α-linolenic acid (ALA)-derived oxylipins in different volumes of soybean oil (pmol/μL).

From: Triacylglycerols are preferentially oxidized over free fatty acids in heated soybean oil

Volume of soybean oil (μL)

1

2

5

10

10 (ref. 6)

10 (ref. 6)

9-HODE

7.20 ± 0.93a

5.23 ± 0.92b

2.70 ± 0.08c

1.76 ± 0.11c

0.18(0.17, 0.23)

0.33 (0.26, 0.80)

13-HODE

12.13 ± 1.38a

7.40 ± 0.10b

4.74 ± 0.30c

2.87 ± 0.45c

0.85 (0.53, 0.86)

1.57 (1.03, 2.10)

9-oxo-ODE

8.90 ± 1.59a

5.18 ± 0.15b

3.11 ± 0.19bc

1.66 ± 0.20c

0.54 (0.26, 1.26)

0.89 (0.72, 1.59)

13-oxo-ODE

8.45 ± 1.32a

4.80 ± 0.75b

3.15 ± 0.57bc

2.09 ± 0.23c

0.07 (0.04, 0.12)

0.28 (0.15, 0.35)

9(10)-EpOME

10.15 ± 1.36a

6.83 ± 0.87b

3.61 ± 0.65c

1.48 ± 0.25c

20.50 (9.75, 40.55)

4.43 (3.14, 5.35)

12(13)-EpOME

21.12 ± 3.79a

16.46 ± 3.88ab

9.41 ± 1.10bc

4.02 ± 0.47c

35.15 (15.30, 56.00)

5.10 (3.51, 6.14)

9,10-DiHOME

20.00 ± 1.61a

15.08 ± 1.15b

11.83 ± 0.22c

8.30 ± 0.51d

15.60 (8.20, 19.75)

23.90 (14.20, 28.80)

12,13-DiHOME

18.79 ± 2.47a

14.57 ± 0.63b

11.53 ± 0.16b

7.90 ± 0.91c

10.25 (5.35, 13.85)

18.80 (10.87, 22.70)

9,10,13-TriHOME

NQ1

NQ

NQ

5.87 ± 7.01

NQ

NQ

9,12,13-TriHOME

NQ

NQ

NQ

6.65 ± 7.85a

NQ

NQ

ΣLA-derived oxylipins

106.76 ± 11.34a

75.55 ± 3.06b

50.09 ± 2.37c

42.59 ± 14.09c

83.14 (39.6, 132.62)

55.30 (33.88, 67.83)

9-HOTrE

0.93 ± 0.12a

0.56 ± 0.03b

0.36 ± 0.01c

0.24 ± 0.04c

0.05 (0.01, 0.05)

0.10 (0.06−0.14)

13-HOTrE

0.79 ± 0.06a

0.42 ± 0.01b

0.31 ± 0.02c

0.20 ± 0.02d

0.02 (0.02, 0.04)

0.16 (0.11−0.24)

ΣALA-derived oxylipins

1.72 ± 0.18a

0.98 ± 0.03b

0.67 ± 0.02c

0.44 ± 0.06c

0.07 (0.03, 0.09)

0.26 (0.17–0.38)

  1. Data are expressed as mean ± SD of n = 3 per oil volume (1, 2, 5, and 10 µL).
  2. a-dDifferent letters within a row are significantly different by one-way ANOVA followed by Tukey’s post-hoc test (p < 0.05).
  3. 1NQ: not quantified because the d4-prostaglandin E2 (d4-PGE2) surrogate standard used to quantify the analyte degraded during hydrolysis.
  4. 2Reference data from Fig. 2 (Experiment 1) in Richardson et al. (2017). Concentrations are expressed as median and interquartile range (25th and 75th percentiles).
  5. 3Reference data from Table 2 (Experiment 2) in Richardson et al. (2017). Concentrations are expressed as median and interquartile range (25th and 75th percentiles).