Table 2 Concentrations of free linoleic acid (LA)-derived and α-linolenic acid (ALA)-derived oxylipins in different volumes of soybean oil (pmol/μL).

Volume of soybean oil (μL)	1	2	5	8
9-HODE	0.37 ± 0.02^a	0.19 ± 0.02^b	0.10 ± 0.01^c	0.09 ± 0.02^c
13-HODE	0.55 ± 0.04^a	0.33 ± 0.06^b	0.18 ± 0.02^c	0.17 ± 0.01^c
9-oxo-ODE	0.32 ± 0.04^a	0.22 ± 0.02^b	0.11 ± 0.01^c	0.09 ± 0.01^c
13-oxo-ODE	ND¹	ND	ND	ND
9(10)-EpOME	0.11 ± 0.10²	0.13 ± 0.01	0.10 ± 0.004	0.08 ± 0.01
12(13)-EpOME	0.45 ± 0.06^a	0.34 ± 0.03^b	0.30 ± 0.02^b	0.29 ± 0.02^b
9,10-DiHOME	0.16 ± 0.01^a	0.12 ± 0.01^b	0.10 ± 0.01^b	0.10 ± 0.003^b
12,13-DiHOME	0.12 ± 0.003^a	0.10 ± 0.02^ab	0.09 ± 0.004^b	0.09 ± 0.004^b
9,10,13-TriHOME	ND	0.06 ± 0.08²	ND	ND
9,12,13-TriHOME	ND	ND	ND	ND
ΣLA-derived oxylipins	2.08 ± 0.08^a	1.49 ± 0.20^b	0.98 ± 0.05^c	0.91 ± 0.02^c
9-HOTrE	ND	ND	0.01 ± 0.004	0.01 ± 0.003
13-HOTrE	ND	ND	ND	ND
ΣALA-derived oxylipins	NA¹	NA	0.01 ± 0.004	0.01 ± 0.003

Data are expressed as mean ± SD of n = 3 per oil volume. In this particular experiment, the peak areas of free 13-HODE and 9-oxo-ODE in the solvent blank extract (n = 1) accounted for 25–69% and 35–108% of the peak areas detected in oil samples, respectively, suggesting background contamination from the silica or HLB columns used to purify the separated oxylipins. We estimated % background contribution in each sample, using the blank peak area. For 13-HODE, the background peak accounted for 66.2 ± 4.4, 56.4 ± 9.2, 41.5 ± 3.6, and 26.2 ± 0.8% of free oxylipins in 1, 2, 5, and 8 µL oil volumes, respectively. For 9-oxo-ODE, the background peak accounted for 93.9 ± 12.3, 66.9 ± 5.0, 53.7 ± 5.6, and 41.6 ± 5.6% of free oxylipins in 1, 2, 5, and 8 µL oil volumes, respectively.
¹ND: not detected, or NA: non-applicable
²One of the three samples was not detected, so it was imputed with LOQ/sqrt(2).
^a–cDifferent letters within a row are significantly different by one-way ANOVA followed by Tukey’s post-hoc test (p < 0.05).

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