Table 2 Phenotypic characteristics of cancer cells and tested biomarkers in 3D peptide hydrogel models.
From: Advances in 3D peptide hydrogel models in cancer research
Cancer cell type | 3D peptide hydrogel type | Cell behaviors in various medium and drug treatment conditions | Biomarkers | References |
|---|---|---|---|---|
MDA-MB-231 breast cancer cells | RADA16, collagen I, and Matrigel | Cells are elongated in collagen I and RADA16 hydrogels but spheroid in Matrigel. Cells in RADA16 hydrogels show reduced migratory ability and tumorgenicity vs. those in collagen I hydrogels and Matrigel. | N/A | Mi et al.25 |
SMMC7221 hepatocellular carcinoma cells | RADA16, collagen I, and Matrigel | Cells form spheroids in RADA16 hydrogels and Matrigel. Cell proliferation is reduced in RADA16 hydrogels vs. collagen I hydrogels and Matrigel. | N/A | Song et al.50 |
A2780, A2780/DDP, and SK-OV-3 ovarian cancer cells | RADA16 and collagen I | Cells exhibit similar adhesion properties and invasion potential in RADA16 and collagen I hydrogels. | N/A | Yang and Zhao21 |
HO-8910PM ovarian cancer cells | RADA16, Matrigel and collagen I | Cells exhibit similar adhesion properties in RADA16 hydrogels, Matrigel and collagen I hydrogels. | Proteins related to adhesion properties: integrin β1, E-cadherin and N-cadherin. | Song et al.51 |
A549 lung adenocarcinoma cells | PuraMatrix and Matrigel | Cells form spheroids and show similar invasion potential in PuraMatrix and Matrigel. | Biomarker of invasion: cortactin | Prina-Mello et al.19 |
MCF-7 breast cancer cells | H9e | Cisplatin reduces cell viability in the hydrogel. | Biomarker of cytoskeletal function: actin; biomarker of proliferation: Ki67; biomarker of apoptosis inhibition: survivin; biomarker of active apoptosis: cleaved caspase-3. | Huang et al.18 |
HNS, UM-SSC-1, and OSC-19 head and neck squamous cell carcinoma cells | H9e | Cells form tumor-like clusters in the hydrogel; ficlatuzumab inhibits cancer cell growth. | Biomarker of epithelial-to-mesenchymal transition: vimentin | Kumar et al.53 |
ATCC CCL-2 HeLa cancer cells | PGmatrix DMEM PGD-006 PepGel LLC | Hydrogels stimulate secretion of in vivo-like extracellular vesicles (i.e., exosomes); spheroids show an in vivo tumor-like morphology. | RNA sequencing | Thippabhotla et al.14 |
