Fig. 3: Myogenic and adipogenic differentiation of bovine FAPs and SCs.
From: Muscle-derived fibro-adipogenic progenitor cells for production of cultured bovine adipose tissue
a Brightfield and immunofluorescence microscopy images of myogenic (top) and adipogenic (bottom panel) differentiation of FAPs and SCs after 96 h (for myogenic) and 14 days (for adipogenic) differentiation in 2D culture vessels, respectively. Green = desmin (myogenic) or BODIPY (adipogenic), blue = Hoechst, scale bar = 200 µm. b Quantified fusion indices of myogenic samples in a. c Expression of muscle-related genes in FAPs and SCs after 96 h of myogenic differentiation, measured by RT-qPCR. Data was normalised against SC control (0 h). d Quantification of BODIPY positive cells in adipogenic samples in a. e Expression of adipogenesis-related genes in FAPs and SCs after 14 days of adipogenic differentiation, measured by RT-qPCR. Data was normalised against FAP control (Day 0). All numerical data is shown as mean ± sd (n = 3); P-values: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.
