Fig. 4: Adipogenic differentiation of FAPs in 3-dimensional hydrogels.
From: Muscle-derived fibro-adipogenic progenitor cells for production of cultured bovine adipose tissue
a Maximum intensity projection confocal microscopy images of control (top) and ADM (bottom panels) treated FAP microfibres after 0, 14, and 28 days of differentiation. Green = BODIPY, blue = Hoechst, scale bar = 100 µm. b Lipid volume per cell, for samples in a (quantified by calculating total lipid volume from BODIPY immunofluorescence, divided by number of nuclei, for three independent images). Condition (i.e., control vs ADM) post-hoc significance in a two-way ANOVA is indicated (condition, time, and their interaction are all statistically significant). c Maximum intensity projection confocal microscopy images of FAP microfibres after 28 days of differentiation. Green = BODIPY, blue = Hoechst, red = PLIN1 (top) or ACC (bottom panels). Scale bar = 40 μm. d Expression of adipogenesis-related genes in FAP microfibres after 0, 7, 14, and 28 days of adipogenic differentiation, measured by RT-qPCR. Data was normalised to the expression on Day 0. Dashed line indicates extent of upregulation after 14 days of 2D differentiation (Fig. 3e). All numerical data is shown as mean ± sd (n = 3); P-values: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.
