Fig. 6: Lipidomic analysis of FAP-derived cultured fat.

a Macroscopic photographs of empty alginate hydrogel, cultured fat (after 28 days of differentiation), and bovine subcutaneous fat. Scale bar = 5 mm. b SEM images of cultured fat after 0 and 28 days of differentiation, and bovine subcutaneous fat. Scale bar = 10 μm. c Absolute quantification of total lipid content (normalised to mass of protein) of cultured fat after 0, 7, and 28 days of differentiation. d Breakdown of relative percentages of fatty acid species within the triglyceride lipid class in cultured fat samples after 0, 7, and 28 days of differentiation, and bovine subcutaneous fat (‘Fat’) and skeletal muscle (‘Muscle’) control samples. Legend denotes C:D numerical annotation (where C indicates number of carbon atoms, and D number of double bonds, in the acyl chain). e Proportion of triglyceride species with acyl chains containing 0 to 6 unsaturations, for three of the samples shown in d. Statistically significant differences to both fat and muscle are indicated. f Proportion of triglyceride species with acyl chains containing 16, 18, 20, or 22 carbon atoms for the same samples shown in e. All numerical data are shown as mean ± sd (n = 3). P-values: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.