Fig. 5: T cells inhibit differentiation of RAW 264.7 macrophages ex vivo. | npj Science of Food

Fig. 5: T cells inhibit differentiation of RAW 264.7 macrophages ex vivo.

From: Obesity, but not high-fat diet, is associated with bone loss that is reversed via CD4+CD25+Foxp3+ Tregs-mediated gut microbiome of non-obese mice

Fig. 5: T cells inhibit differentiation of RAW 264.7 macrophages ex vivo.The alternative text for this image may have been generated using AI.

a Study design. T cells obtained from CD, HIO, and NO were cocultured with RAW264.7 macrophages for 9 days. b TRAP staining. c Total RNA was extracted from the RAW264.7 macrophages, and the relative expression of Rankl, Opg, Rankl/Opg and Trap was determined. d–e Assessment of cytokines of IL-10 and TGF-β levels in cell supernatants. n = 3 per group. Data are presented as means ± SEM. Two-way ANOVA was used to analyze the significance of differences. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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