Fig. 1: Proliferation and differentiation of large yellow croaker PSCs and PADSCs.

a Diagrams showing that epaxial muscles and coelom walls of large yellow croaker were used to isolate the primary culture of PSCs and PADSCs respectively. Scale bars: 80 µm. b Primary cultures at different time points and passage 3 of PSCs and PADSCs. Scale bars: 80 µm; c The growth curves of PSCs and PADSCs at passage 3 were analyzed by CCK-8 method with three replicates. d Representative images of immunofluorescence staining of PSCs at passage 3. Green: Pax7 or MyoD1; Blue: Hoechst 33342 for nuclear staining. Scale bars: 80 µm. e Average percentages of Pax7- and MyoD1-positive cells derived from image d with five replicates. Box indicates IQR; whisker indicates min or max; plus shows mean. f Representative images of bright-field and fluorescence staining of PSCs during myogenic differentiation at day 0, 3, 6. Green: Desmin; Blue: Hoechst 33342; Scale bars: 80 µm. g Myotube fusion index counted from image i with five replicates. Box: IQR; Whiskers: min or max; Plus: mean. h Image of immunofluorescence staining of PADSCs at passage 3. Green: HoxC9; Blue: Hoechst 33342. Scale bars: 80 µm. i Average percentage of HoxC9-positive cells counted from image f with 5 replicates. Box: IQR; Whisker: min or max; Plus: mean. j Representative images of bright-field and oil red O staining of PADSCs during adipogenic differentiation at day 0, 1, 6. Lipid droplets in red were stained by oil red O and nuclei in blue were stained by hematoxylin. Scale bars: 40 µm.