Fig. 3: Effects of supporting materials of 3D-scaffold on fish cell adhesion and growth.
From: Tissue-like cultured fish fillets through a synthetic food pipeline
a Average cell viabilities in different supporting materials including porcine gelatin (PG), fish gelatin (FG), Hyaluronic acid (HA), silk fibroin (SF), and chitosan. Statistical analysis was performed on relevant data using Student’s two-tailed t-test. Error bars for three replicates indicated s.d. NS: not significant; *P < 0.05. b, c Gel point (b) and viscosity (c) of supporting materials with different ratios of gelatin basis (5/10%) and 1% sodium alginate (SA). The square and triangle symbols in b represented G’ and G” respectively. d Calcein-AM fluorescence images of PSCs treated with different concentrations of CaCl2 for different times. The non-adherent PSCs were spherical, while the adherent PSCs became spindle-shaped and gradually underwent proliferation. Scale bar, 50 μm. e, f Elastic modulus of scaffolds (e) and cell adhesion rates of PSCs (f) treated with different concentrations of CaCl2 for different times. The experiments were repeated for three times and statistical analysis was performed on relevant data using Student’s two-tailed t-test. Error bars indicated s.d. **P < 0.005, ****P < 0.0001. g Average number of PSCs measured with CCK-8 kit at day 5 of 3D culture with three replicates. Error bars indicated s.d. **P < 0.005, ***P < 0.0005, ****P < 0.0001. h Average myotube fusion index in different 3D Culture Conditions as indicated. R: Repsox; L: LY411575. Statistical analysis was performed on relevant data using Student’s two-tailed t-test. Error bars for three replicates indicated s.d. **P < 0.005, ***P < 0.0005.
