Fig. 4: Muscle tissue formation under guidance of biomimetic model of scaffold.
From: Tissue-like cultured fish fillets through a synthetic food pipeline
a Schematic diagram of construction of biomimetic 3D-printed model based on native large yellow croaker muscle tissue. b Micro-CT images of native fish epaxial muscle. Blue: Intermuscular filler tissues; Orange: Muscle tissues; Muscle scaffold model was designed from simplified native muscle texture. Scale bar: 1 mm. c Calcein-AM fluorescence images of PSCs in scaffolds at day 3 and 10 days of proliferation. Scale bar, 100 μm. d Representative fluorescence image of myobubes in 3D culture at days 7 of differentiation. The PSCs in scaffolds were cultured in proliferation medium for 10 days, and then transferred to differentiation medium for 7 days. Green: Desmin; Blue: DAPI. Scale bar, 100 μm. e Growth curve of PSCs in optimized gelatin-based scaffold. Error bars for three replicates indicate s.d.
