Fig. 6: Comparison of gene programs involved in lipid metabolism between the HLW and LLW groups. | npj Science of Food

Fig. 6: Comparison of gene programs involved in lipid metabolism between the HLW and LLW groups.

From: Single-nucleus and bulk RNA sequencing reveal cellular and transcriptional mechanisms underlying lipid dynamics in high marbled pork

Fig. 6: Comparison of gene programs involved in lipid metabolism between the HLW and LLW groups.

a Volcano plot showing log2-fold changes in exons of RNA-Seq gene bodies in LDM of the HLW and LLW groups (n = 4) and the corresponding significance values displayed as log10 (P value). The transverse and vertical dotted lines indicate the cut-off value for differential expression (P < 0.05 and abs (log2-fold changes) >1). In total, 534 and 500 genes that had upregulated (red) or downregulated (blue) expression levels were identified. b TPM values of preadipocyte-related genes, adipogenic master genes, mature adipocyte marker genes, and lipid metabolism-related genes in different groups (n = 4). c t-SNE and violin plot displaying the expression of differential lipid metabolism-related genes in LDM nuclei. d KEGG enrichment analysis of genes in LDM. e Heatmap showing the relative expression of glycerolipid pathway-related genes derived from the RNA-seq dataset. f Selected fatty acid metabolic reactions from KEGG, with indications of quantified lipid classes and acyl chains (circles) and genes (rectangles) significantly regulated compared with LLW group. Statistical analysis was performed using two-tailed Student’s t-test.

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