Fig. 1: Strategies for the biosynthesis of D-psicose.
From: Awakening the natural capability of psicose production in Escherichia coli
a The current industrial method for D-psicose production leads to limited yield (~50%) due to a positive ΔG’°. b In the proposed biosynthetic pathway of D-psicose, the dephosphorylation step thermodynamically drives production forwards due to a large negative ΔG’m under cellular reactant concentrations of 1 mM. c The proposed pathway for the biosynthetic production of D-psicose in E. coli. Deleted steps are in blue. Overexpressed steps are in red. PTS, the phosphotransferase system; AlsE, D-allulose 6-phosphate 3-epimerase, HxpB hexitol phosphatase B.
