Table 1 List of key strains used in this study.
From: Awakening the natural capability of psicose production in Escherichia coli
Strain no. | E. coli strain | Key genotype | Plasmid | Plasmid contents |
|---|---|---|---|---|
1 | AL3756 | ΔpfkA Δzwf ΔrpiB | pAL2001 | PLlacO1:alsE-hxpB |
2 | AL3990 | 1 + ΔmanA | pAL2001 | PLlacO1:alsE-hxpB |
3 | AL3990 | 1 + ΔmanA | pAL2247 | PgadB:alsE-hxpB |
4 | AL3990 | 1 + ΔmanA | pAL2247 pAL2264 | PgadB:alsE-hxpB, PLlacO1:galP-glk |
5 | AL4186 | 2 + Δpgm | pAL2247 pAL2264 | PgadB:alsE-hxpB, PLlacO1:galP-glk |
6 | AL4186 | 2 + Δpgm | pAL2247 pAL2264 pAL2182 | PgadB:alsE-hxpB, PLlacO1:galP-glk, Ptet:dcas9 pTargetF-no target |
7 | AL4186 | 2 + Δpgm | pAL2247 pAL2264 pAL2188 | PgadB:alsE-hxpB, PLlacO1:galP-glk, Ptet:dcas9 pTargetF-pfkB |
