Fig. 4: Analysis of metabolome in muscle of PPM1J knockdown.

A–D The OPLS-DA score scatter plots result of the positive and negative ion mode in PPM1J knockdown muscle. B–F Statistics (B), heatmap (C), chemical taxonomy (D) of differential metabolites in PPM1J knockdown muscle. E Relative meat flavor-related metabolite content in PPM1J knockdown muscle. F KEGG pathway analysis of differential metabolites in PPM1J knockdown muscle. In panel E, results are shown as mean + SEM, statistical significance of differences between means was assessed using paired t-tests. (*P < 0.05; **P < 0.01).