Fig. 2: Feasibility verification. | npj Science of Food

Fig. 2: Feasibility verification.

From: Endonuclease V activated Pyrococcus furiosus Argonaute for the detection of food contaminated bacteria

Fig. 2

a Sequence cut diagram of VPN. P: phosphorylation. F: FAM fluorophore. Q: BHQ1 quencher. b Fluorescence spectra of different enzyme systems. c Fluorescence imaging of different systems at 365 nm ultraviolet light. ‘+’ represents the presence of PfAgo or Endo V or Endo III, and ‘-’ represents the absence of PfAgo or Endo V or Endo III. d Native polyacrylamide gel electrophoresis (15%). (line 1:amplification product, line 2: Endo V cleave amplification product, line 3: in the absence of MB, PfAgo cleave Endo V cleavage products, line 4: PfAgo cleave Endo V cleavage products with MB, line 5: MB). (source: Microsoft Office PowerPoint, Origin).

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