Fig. 4: Use VPN to detect a single foodborne pathogen. | npj Science of Food

Fig. 4: Use VPN to detect a single foodborne pathogen.

From: Endonuclease V activated Pyrococcus furiosus Argonaute for the detection of food contaminated bacteria

Fig. 4

a Detection of DNA by VPN. b A linear regression equation with a target gene concentration of 0.2-800 ng/μL. c The repeatability of VPN. d Stability of VPN. VPN for the detection of the four food-borne pathogens : E. coli O157:H7 (e), S. aureus (f), S. typhimurium (g) and C. sakazakii (h),under different types of interference. The concentration of non-target bacteria is 100-fold higher than that of target bacteria. Date are presented as mean ± s.d., n = 3. P value were calculated using two-sided one-way ANOVA post-Dunnett’s test;***p < 0.001. i–l The sensitivity of VPN to detect four food-borne pathogens : E.coli O157:H7 (i), S.aureus (j), S. typhimurium (k) and C. sakazakii (l). (source: Microsoft Office PowerPoint, Origin).

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