Fig. 6: Differential metabolites and their functional analysis in the muscle of silver carp during transportation.

A The proportion of all metabolites. B Volcano plot of muscle metabolites. C OPLS-DA of muscle metabolites. D The Venn diagrams of differential metabolites. E The diagrams of KEGG pathways enrichment. T6 vs T3: 1. Cell cycle – yeast 2. PI3K−Akt signaling pathway 3. mTOR signaling pathway 4. Olfactory transduction 5. FoxO signaling pathway 6. cGMP−PKG signaling pathway 7. Parathyroid hormone synthesis, secretion, and action 8. Regulation of lipolysis in adipocytes 9. Aldosterone synthesis and secretion 10. Oxidative phosphorylation 11. Renin secretion 12. Thermogenesis 13. Taste transduction 14. Pentose and glucuronate interconversions 15. ABC transporters 16. Inositol phosphate metabolism 17. Purine metabolism 18. Ascorbate and aldarate metabolism 19. Lysine degradation 20. Amino sugar and nucleotide sugar metabolism. T12 vs T3: 1. Lysosome 2. FoxO signaling pathway 3. Long−term depression 4. Circadian entrainment 5. Gap junction 6. Taurine and hypotaurine metabolism 7. Thermogenesis 8. Glucagon signaling pathway 9. Citrate cycle (TCA cycle) 10. Platelet activation 11. AMPK signaling pathway 12. Carbon fixation in photosynthetic organisms 13. Pyruvate metabolism 14. Protein digestion and absorption 15. Ferroptosis 16. Taste transduction 17. Carbon fixation pathways in prokaryotes 18. Butanoate metabolism 19. Glyoxylate and dicarboxylate metabolism 20. Carbon metabolism. T24 vs T3: 1. Long−term depression 2. GABAergic synapse 3. Circadian entrainment 4. Gap junction 5. Synaptic vesicle cycle 6. Thermogenesis 7. Alanine, aspartate, and glutamate metabolism 8. Ferroptosis 9. Taste transduction 10. Fructose and mannose metabolism 11. Galactose metabolism 12. Protein digestion and absorption 13. Ascorbate and aldarate metabolism 14. Aminoacyl−tRNA biosynthesis 15. ABC transporters 16. Phosphotransferase system (PTS) 17. Phenylalanine metabolism 18. Amino sugar and nucleotide sugar metabolism 19. Carbon metabolism 20. Biosynthesis of amino acids. PIM stands for positive ion mode, and NIM stands for negative ion mode. T3, T6, T12, and T24 represent samples under transportation for 3, 6, 12, and 24 h.