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Functional evaluation of wax-based oleogels as solid fat replacers for designing low saturated fat plant-based meat analogues
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  • Published: 16 January 2026

Functional evaluation of wax-based oleogels as solid fat replacers for designing low saturated fat plant-based meat analogues

  • Young Seo Park1,
  • Sungmin Jeong2,3 &
  • Suyong Lee1,2 

npj Science of Food , Article number:  (2026) Cite this article

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We are providing an unedited version of this manuscript to give early access to its findings. Before final publication, the manuscript will undergo further editing. Please note there may be errors present which affect the content, and all legal disclaimers apply.

Subjects

  • Biochemistry
  • Chemistry

Abstract

Meat analogues have gained global attention as consumer demand increases for healthier and more sustainable food products. However, research aimed at replacing solid fats in meat analogues to lower saturated fat content remains very limited. Canola oil was structured with three natural waxes (candelilla wax, carnauba wax, and beeswax) and their potentials as solid fat replacers were evaluated for low saturated fat meat analogues. The wax-based oleogels retained solid fat at higher temperatures compared to coconut oil. Candelilla wax oleogels showed the highest hardness at room temperature. Upon melting, carnauba wax oleogels exhibited the highest viscosity and the greatest sensitivity to temperature changes, as evidenced by their highest activation energy. Replacement of coconut oil with wax-based oleogels did not significantly alter the visual appearance of meat analogues. Meat analogues with oleogels had significantly lower cooking loss, especially with carnauba wax oleogels. The hardness of coconut oil and oleogels was correlated to that of the corresponding meat analogues (R2 = 0.76). Additionally, oleogel-based analogues had a much healthier fatty acid profile, with lower saturated and higher unsaturated fat content, closely resembling canola oil. Therefore, this study demonstrated that wax-based oleogels were promising solid fat alternatives for developing plant-based meat analogues with enhanced cooking performance and healthier fatty acid composition.

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Data availability

Data will be made available on request.

References

  1. Lee, H. J., Yong, H. I., Kim, M., Choi, Y.-S. & Jo, C. Status of meat alternatives and their potential role in the future meat market — a review. Asian Australas. J. Anim. Sci. 33, 1533–1543 (2020).

    Google Scholar 

  2. Malila, Y. et al. Current challenges of alternative proteins as future foods. npj Sci. Food 8, 53 (2024).

    Google Scholar 

  3. Bohrer, B. M. An investigation of the formulation and nutritional composition of modern meat analogue products. Food Sci. Hum. Wellness 8, 320–329 (2019).

    Google Scholar 

  4. Sun, Y. et al. Preparation and properties of high-soluble wheat gluten protein-based meat analogues. J. Sci. Food Agric. 104, 42–50 (2024).

    Google Scholar 

  5. Zhu, H.-G. et al. Potential of preparing meat analogue by functional dry and wet pea (Pisum sativum) protein isolate. LWT 148, 111702 (2021).

    Google Scholar 

  6. Wang, M. & Zhao, R. A review on nutritional advantages of edible mushrooms and its industrialization development situation in protein meat analogues. J. Future Foods 3, 1–7 (2023).

    Google Scholar 

  7. Lee, J.-S., Choi, I. & Han, J. Construction of rice protein-based meat analogues by extruding process: effect of substitution of soy protein with rice protein on dynamic energy, appearance, physicochemical, and textural properties of meat analogues. Food Res. Int. 161, 111840 (2022).

    Google Scholar 

  8. Aguilera, J. M., Rossi, F., Hiche, E. & Chichester, C. O. Development and evaluation of an extrusion-texturized peanut protein. J. Food Sci. 45, 246–254 (1980).

    Google Scholar 

  9. Fernandes, W. D., Pinto, F. R., Barroso, S. & Gil, M. M. Development, characterisation, and consumer acceptance of an innovative vegan burger with seaweed. Sustainability 15, 10869 (2023).

    Google Scholar 

  10. Teng, C. & Campanella, O. H. A plant-based animal fat analog produced by an emulsion gel of alginate and pea protein. Gels 9, 393 (2023).

    Google Scholar 

  11. Bogueva, D. & McClements, D. J. Safety and nutritional risks associated with plant-based meat alternatives. Sustainability 15, 14336 (2023).

    Google Scholar 

  12. Guo, J., Huang, Y., Gu, X. & Meng, Z. Spirulina platensis protein-based emulsion gel as fat substitute in meat analogs: evaluation performance across post-processing. Food Chem. 463, 141414 (2025).

    Google Scholar 

  13. Lee, J. et al. Physicochemical and sensory properties of plant-based meat patties using oil-in-water emulsion. Food Biosci. 56, 103084 (2023).

    Google Scholar 

  14. Temkov, M. & Mureșan, V. Tailoring the structure of lipids, oleogels and fat replacers by different approaches for solving the trans-fat issue—A review. Foods 10, 1376 (2021).

    Google Scholar 

  15. Silva, T. J., Barrera-Arellano, D. & Ribeiro, A. P. B. Oleogel-based emulsions: concepts, structuring agents, and applications in food. J. Food Sci. 86, 2785–2801 (2021).

    Google Scholar 

  16. EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS) Scientific opinion on the re-evaluation of carnauba wax (E 903) as a food additive. EFSA J. 10, 2880 (2012).

    Google Scholar 

  17. EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS) Scientific opinion on the re-evaluation of candelilla wax (E 902) as a food additive. EFSA J. 10, 2946 (2012).

    Google Scholar 

  18. EFSA Panel on Food Additives Flavourings, processing aids and materials in contact with food (AFC). Beeswax (E 901) as a glazing agent and as carrier for flavours. EFSA J. 615, 1–28 (2007).

    Google Scholar 

  19. Alvarez-Ramirez, J., Vernon-Carter, E. J., Carrera-Tarela, Y., Garcia, A. & Roldan-Cruz, C. Effects of candelilla wax/canola oil oleogel on the rheology, texture, thermal properties and in vitro starch digestibility of wheat sponge cake bread. LWT 130, 109701 (2020).

    Google Scholar 

  20. Mert, B. & Demirkesen, I. Reducing saturated fat with oleogel/shortening blends in a baked product. Food Chem. 199, 809–816 (2016).

    Google Scholar 

  21. Jeong, S., Lee, S. & Oh, I. Development of antioxidant-fortified oleogel and its application as a solid fat replacer to muffin. Foods 10, 3059 (2021).

    Google Scholar 

  22. Igenbayev, A. et al. Effect of using oleogel on the physicochemical properties, sensory characteristics, and fatty acid composition of meat patties. Foods 13, 3849 (2024).

    Google Scholar 

  23. Gómez-Estaca, J. et al. Characterization of ethyl cellulose and beeswax oleogels and their suitability as fat replacers in healthier lipid pâtés development. Food Hydrocoll. 87, 960–969 (2019).

    Google Scholar 

  24. Tarté, R. et al. High-oleic and conventional soybean oil oleogels structured with rice bran wax as alternatives to pork fat in mechanically separated chicken-based bologna sausage. LWT 131, 109659 (2020).

    Google Scholar 

  25. Czapalay, E. & Marangoni, A. Functional properties of oleogels and emulsion gels as adipose tissue mimetics. Trends Food Sci. Technol. 153, 104753 (2024).

    Google Scholar 

  26. Sonwai, S., Rungprasertphol, P., Nantipipat, N., Tungvongcharoan, S. & Laiyangkoon, N. Characterization of coconut oil fractions obtained from solvent fractionation using acetone. J. Oleo Sci. 66, 951–961 (2017).

    Google Scholar 

  27. Scharfe, M., Niksch, J. & Flöter, E. Influence of minor oil components on sunflower, rice bran, candelilla, and beeswax oleogels. Eur. J. Lipid Sci. Technol. 124, 2100068 (2022).

    Google Scholar 

  28. Dassanayake, L. S. K., Kodali, D. R., Ueno, S. & Sato, K. Physical properties of rice bran wax in bulk and organogels. J. Am. Oil Chem. Soc. 86, 1163–1173 (2009).

    Google Scholar 

  29. Muhidinov, Z. K. et al. Effect of temperature on the intrinsic viscosity and conformation of different pectins. Polym. Sci. Ser. A 52, 1130–1137 (2010).

    Google Scholar 

  30. Ali, S. A. K. Newtonian fluids in piping systems. Int. J. Sci. Res. 9, 975–979 (2020).

    Google Scholar 

  31. Kamal, M. R. & Mutel, A. Rheological properties of suspensions in Newtonian and non-Newtonian fluids. J. Polym. Eng. 5, 293–382 (1985).

    Google Scholar 

  32. Lee, D. Y. et al. Current technologies and future perspective in meat analogs made from plant, insect, and mycoprotein materials: a review. Food Sci. Anim. Resour. 44, 1–18 (2024).

    Google Scholar 

  33. Pastrana-Pastrana, ÁJ. et al. Plant proteins, insects, edible mushrooms and algae: more sustainable alternatives to conventional animal protein. J. Future Foods 5, 248–256 (2025).

    Google Scholar 

  34. Lim, J., Hwang, H.-S. & Lee, S. Oil-structuring characterization of natural waxes in canola oil oleogels: rheological, thermal, and oxidative properties. Appl. Biol. Chem. 60, 17–22 (2017).

    Google Scholar 

  35. Çakır, M., Özer, C. O. & Var, G. B. Utilization of sunflower oil-based oleogel for deep-fried coated chicken products. J. Oleo Sci. 72, 399–407 (2023).

    Google Scholar 

  36. Franco, D. et al. Strategy towards replacing pork backfat with a linseed oleogel in frankfurter sausages and its evaluation on physicochemical, nutritional, and sensory characteristics. Foods 8, 366 (2019).

    Google Scholar 

  37. Kim, M., Hwang, H.-S., Jeong, S. & Lee, S. Utilization of oleogels with binary oleogelator blends for filling creams low in saturated fat. LWT 155, 112972 (2022).

    Google Scholar 

  38. Sheard, P. R., Nute, G. R. & Chappell, A. G. The effect of cooking on the chemical composition of meat products with special reference to fat loss. Meat Sci. 49, 175–191 (1998).

    Google Scholar 

  39. Wang, P. et al. Quality characteristics and storage stability of frying steak utilizing wax-based Korean pine seed oil. Foods 13, 1099 (2024).

    Google Scholar 

  40. Bourne, M. C. Food texture and viscosity: concept and measurement, 184–188 (Academic Press, 2002).

  41. De Marchi, M., Costa, A., Pozza, M., Goi, A. & Manuelian, C. L. Detailed characterization of plant-based burgers. Sci. Rep. 11, 2049 (2021).

    Google Scholar 

  42. Jang, A., Bae, W., Hwang, H.-S., Lee, H. G. & Lee, S. Evaluation of canola oil oleogels with candelilla wax as an alternative to shortening in baked goods. Food Chem. 187, 525–529 (2015).

    Google Scholar 

  43. Lim, J., Jeong, S., Oh, I. K. & Lee, S. Evaluation of soybean oil–carnauba wax oleogels as an alternative to high saturated fat frying media for instant fried noodles. LWT 84, 788–794 (2017).

    Google Scholar 

  44. Oh, I., Lee, J., Lee, H. G. & Lee, S. Feasibility of hydroxypropyl methylcellulose oleogel as an animal fat replacer for meat patties. Food Res. Int. 122, 566–572 (2019).

    Google Scholar 

  45. Kong, W., Wang, L., Xu, H. & Liu, D. Effects of lecithin/sorbitol monostearate–canola oil oleogel as animal fat replacer on the fatty acid composition and physicochemical properties of lamb sausage. J. Food Process. Preserv. 2023, 2567854 (2023).

    Google Scholar 

  46. Liu, L. et al. A comprehensive review: impact of oleogel application on food texture and sensory properties. Food Sci. Nutr. 12, 3849–3862 (2024).

    Google Scholar 

  47. Yilmaz, E. & Öğütcü, M. The texture, sensory properties and stability of cookies prepared with wax oleogels. Food Funct. 6, 1194–1204 (2015).

    Google Scholar 

  48. Martínez-Velasco, A., Trujillo-Ramírez, D., Bustos-Vázquez, G. & Cervantes-Arista, C. The use of candelilla wax/canola oil oleogel in the formulation of sponge cake bread improves morphostructural and sensory properties. Discov. Food 4, 160 (2024).

    Google Scholar 

  49. Gao, Y. et al. Preparation of rapeseed oil oleogels based on beeswax and its application in beef heart patties to replace animal fat. LWT 149, 111986 (2021).

    Google Scholar 

  50. Lee, D., Jeong, S., Yun, S. & Lee, S. Artificial intelligence-based prediction of the rheological properties of hydrocolloids for plant-based meat analogues. J. Sci. Food Agric. 104, 5114–5123 (2024).

    Google Scholar 

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Acknowledgements

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through the High Value-added Food Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (RS-2024-00509810), and also by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (RS-2022-NR069007).

Author information

Authors and Affiliations

  1. Department of Food Science & Biotechnology and Sejong University, Seoul, Republic of Korea

    Young Seo Park & Suyong Lee

  2. Carbohydrate Bioproduct Research Center, Sejong University, Seoul, Republic of Korea

    Sungmin Jeong & Suyong Lee

  3. Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu, Republic of Korea

    Sungmin Jeong

Authors
  1. Young Seo Park
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  2. Sungmin Jeong
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  3. Suyong Lee
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Contributions

Y.S.P. conducted formal analyses and study design, interpreted the data, and drafted and edited the manuscript. S.J. interpreted the data, supervised the study, and edited the manuscript. S.L. contributed to conceptualisation, supervised, edited the manuscript and acquired funding.

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Correspondence to Suyong Lee.

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Park, Y.S., Jeong, S. & Lee, S. Functional evaluation of wax-based oleogels as solid fat replacers for designing low saturated fat plant-based meat analogues. npj Sci Food (2026). https://doi.org/10.1038/s41538-026-00713-x

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  • Received: 28 August 2025

  • Accepted: 05 January 2026

  • Published: 16 January 2026

  • DOI: https://doi.org/10.1038/s41538-026-00713-x

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