Abstract
This study developed a novel gelatin-stabilized nano-silver particle/agar hydrogel (AgNP hydrogel) and investigated its color rendering mechanism for monitoring the freshness of braised chicken. The results showed that the color of AgNP hydrogel shifted from brown to gray-white within 3 days of storage of braised chicken, and its application extended the shelf life of unpackaged braised chicken meat from 4 d to 6 d at 4 °C (P < 0.05). The results of circular dichroism (CD), particle size, zeta potential, and UV indicated that Cl- on the surface of braised chicken could react with Ag+ on the surface of AgNP, resulting in the formation of AgCl particles during storage. These AgCl particles were subsequently transformed into black Ag2S particles, causing the visual color shift of AgNP to gray-white. Overall, the AgNP hydrogel could provide a new method for the freshness assessment of cooked meat products.

Data availability
All data generated or analyzed during this study are included in this published article and its supplementary information files.
References
Du, C., Qi, J., Yang, C., Zhang, Q. & Liu, D. Enrichment of taste and aroma perceptions in chicken meat stewed in braised soup used repeatedly. J. Food Sci. 87, 2563–2577 (2022).
Qi, J. et al. Enrichment of taste and aroma compounds in braised soup during repeated stewing of chicken meat. Lwt-Food Sci. Technol. 168, https://doi.org/10.1016/j.lwt.2022.113926 (2022).
Zhou, Y., Li, Z., Chen, Y., Fang, H. & Wang, S. Effects of rosemary and ginger on the storage quality of western-style smoked sausage. J. Food Process. Preserv. 45, e15634 (2021).
Chen, X. et al. Effect of modified atmosphere packaging on shelf life and bacterial community of roast duck meat. Food Res. Int. 137, 109645 (2020).
Yuan, D., Hao, X., Liu, G., Yue, Y. & Duan, J. A novel composite edible film fabricated by incorporating W/O/W emulsion into a chitosan film to improve the protection of fresh fish meat. Food Chem. 385, 132647 (2022).
Chen, M. et al. Intelligent biogenic pH-sensitive and amine-responsive color-changing label for real-time monitoring of shrimp freshness. J. Sci. Food Agric. 103, 7798–7808 (2023).
Zhang, J. et al. Fabrication and characterization of polyvinyl alcohol/sodium alginate/zein/chitosan bilayer film for dynamic visualization of pork quality. Int. J. Biol. Macromolecules, 125065 https://doi.org/10.1016/j.ijbiomac.2023.125065 (2023).
Li, C. et al. Biobased intelligent food-packaging materials with sustained-release antibacterial and real-time monitoring ability. ACS Appl. Mater. Interfaces 15, 37966–37975 (2023).
Chen, A. et al. Rapid determination of chloride using silver triangular nanoplates. Anal. Lett. 48, 2223–2233 (2015).
Dunn, K. & Edwards-Jones, V. The role of Acticoat™ with nanocrystalline silver in the management of burns. Burns 30, S1–S9 (2004).
Semchuk, O. Y., Biliuk, A., Havryliuk, O. & Biliuk, A. Kinetic theory of electroconductivity of metal nanoparticles in the condition of surface plasmon resonance. Appl. Surf. Sci. Adv. 3, 100057 (2021).
Kravets, V. G., Kabashin, A. V., Barnes, W. L. & Grigorenko, A. N. Plasmonic surface lattice resonances: a review of properties and applications. Chem. Rev. 118, 5912–5951 (2018).
Azizi-Lalabadi, M., Garavand, F. & Jafari, S. M. Incorporation of silver nanoparticles into active antimicrobial nanocomposites: Release behavior, analyzing techniques, applications and safety issues. Adv. Colloid Interface Sci. 293, 102440 (2021).
Logaranjan, K., Raiza, A. J., Gopinath, S. C., Chen, Y. & Pandian, K. Shape-and size-controlled synthesis of silver nanoparticles using Aloe vera plant extract and their antimicrobial activity. Nanoscale Res. Lett. 11, 1–9 (2016).
Donga, S. & Chanda, S. Facile green synthesis of silver nanoparticles using Mangifera indica seed aqueous extract and its antimicrobial, antioxidant and cytotoxic potential (3-in-1 system). Artif. cells, Nanomed. Biotechnol. 49, 292–302 (2021).
Tavares, T. D. et al. Activity of specialized biomolecules against gram-positive and gram-negative bacteria. Antibiotics 9, 314 (2020).
Han, Y. et al. Effect of oxidation on surface-enhanced Raman scattering activity of silver nanoparticles: a quantitative correlation. Anal. Chem. 83, 5873–5880 (2011).
Ma, Y., Shen, X. F., Liu, F. & Pang, Y. H. Colorimetric detection toward halide ions by a silver nanocluster hydrogel. Talanta 211, https://doi.org/10.1016/j.talanta.2020.120717 (2020).
Mathew, S., Snigdha, S., Mathew, J. & Radhakrishnan, E. K. Biodegradable and active nanocomposite pouches reinforced with silver nanoparticles for improved packaging of chicken sausages. Food Packaging Shelf Life 19, 155–166 (2019).
Bekhit, A. E. D. A., Giteru, S. G., Holman, B. W. & Hopkins, D. L. Total volatile basic nitrogen and trimethylamine in muscle foods: Potential formation pathways and effects on human health. Compr. Rev. Food Sci. Food Saf. 20, 3620–3666 (2021).
Kim, N. H., Kim, H. W., Moon, H. & Rhee, M. S. Sodium chloride significantly enhances the bactericidal actions of carvacrol and thymol against the halotolerant species Escherichia coli O157: H7, Listeria monocytogenes, and Staphylococcus aureus. LWT Food Sci. Technol. 122, 109015 (2020).
Katiyo, W., de Kock, H. L., Coorey, R. & Buys, E. M. Sensory implications of chicken meat spoilage in relation to microbial and physicochemical characteristics during refrigerated storage. LWT Food Sci. Technol. 128, 109468 (2020).
Zeng, F., Ye, Y., Liu, J. & Fei, P. Intelligent pH indicator composite film based on pectin/chitosan incorporated with black rice anthocyanins for meat freshness monitoring. Food Chem. X 17, 100531 (2023).
Huang, Z. et al. Antimicrobial efficacy and mechanisms of silver nanoparticles against Phanerochaete chrysosporium in the presence of common electrolytes and humic acid. J. Hazard. Mater. 383, 121153 (2020).
Yu, J., Tian, H., Huang, M. & Xu, X. Facile synthesis of Ag NP films via evaporation-induced self-assembly and the BA-sensing properties. Foods 12, 1285 (2023).
Lavanya, K., Kalaimurugan, D., Shivakumar, M. S. & Venkatesan, S. Gelatin stabilized silver nanoparticle provides higher antimicrobial efficiency as against chemically synthesized silver nanoparticle. J. Clust. Sci. 31, 265–275 (2020).
Zein, R., Alghoraibi, I., Soukkarieh, C., Ismail, M. T. & Alahmad, A. Influence of polyvinylpyrrolidone concentration on properties and anti-bacterial activity of green synthesized silver nanoparticles. Micromachines 13, 777 (2022).
Pérez-Marroquín, X. A. et al. Green synthesis of silver nanoparticles for preparation of gelatin films with antimicrobial activity. Polymers 14, 3453 (2022).
Zhao, Y. et al. Comparative study on the effect of different salts on surimi gelation and gel properties. Food Hydrocolloids, 108982 https://doi.org/10.1016/j.foodhyd.2023.108982 (2023).
Sow, L. C. & Yang, H. Effects of salt and sugar addition on the physicochemical properties and nanostructure of fish gelatin. Food Hydrocoll. 45, 72–82 (2015).
Milosavljevic, V. et al. Alternative synthesis route of biocompatible polyvinylpyrrolidone nanoparticles and their effect on pathogenic microorganisms. Mol. Pharmaceutics 14, 221–233 (2017).
Phoonsawat, K., Ratnarathorn, N., Henry, C. S. & Dungchai, W. A distance-based paper sensor for the determination of chloride ions using silver nanoparticles. Analyst 143, 3867–3873 (2018).
Zeng, Y., Wu, X., Yang, H., Zong, M. & Lou, W. 1, 4-α-glucosidase from fusarium solani for controllable biosynthesis of silver nanoparticles and their multifunctional applications. Int. J. Mol. Sci. 24, 5865 (2023).
Zhao, J. et al. Ionic-strength-dependent effect of suspended sediment on the aggregation, dissolution and settling of silver nanoparticles. Environ. Pollut. 279, 116926 (2021).
Qi, J. et al. Effect of short-term frozen storage of raw meat on aroma retention of chicken broth: a perspective on physicochemical properties of broth. LWT Food Sci. Technol. 162, 113480 (2022).
Lei, Y., Zhang, Y., Cheng, Y., Huang, J. & Huang, M. Monitoring and identification of spoilage-related microorganisms in braised chicken with modified atmosphere packaging during refrigerated storage. Food Sci. Hum. Wellness 12, 28–34 (2023).
Zhai, X. et al. A colorimetric hydrogen sulfide sensor based on gellan gum-silver nanoparticles bionanocomposite for monitoring of meat spoilage in intelligent packaging. Food Chem. 290, 135–143 (2019).
Yang, Y. et al. Environmental behavior of silver nanomaterials in aquatic environments: an updated review. Sci. Total Environ. 167861 https://doi.org/10.1016/j.scitotenv.2023.167861 (2023).
Estrada-Mendoza, T. A., Willett, D. & Chumanov, G. Light absorption and scattering by silver/silver sulfide hybrid nanoparticles. J. Phys. Chem. C. 124, 27024–27031 (2020).
Feng, D., Zhang, R., Zhang, M., Fang, A. & Shi, F. Synthesis of eco-friendly silver nanoparticles using glycyrrhizin and evaluation of their antibacterial ability. Nanomaterials 12, 2636 (2022).
Liu, Y. et al. A fluorescent pH probe for evaluating the freshness of chicken breast meat. Food Chem. 384, 132554 (2022).
Sangaonkar, G. M., Desai, M. P., Dongale, T. D. & Pawar, K. D. Selective interaction between phytomediated anionic silver nanoparticles and mercury leading to amalgam formation enables highly sensitive, colorimetric and memristor-based detection of mercury. Sci. Rep. 10, 2037 (2020).
Acknowledgements
This work was supported by the National Natural Science Foundation of China (32001724), the Outstanding Young Teachers Cultivation Program for Young and Middle-aged Teachers Cultivation Action in Universities in Anhui Province (YQZD2023015), Anhui Graduate Academic Innovation Project (2022xscx047), and the University-level Innovation and Entrepreneurship Training Program for College Students of Anhui Agricultural University (X202310364007).
Author information
Authors and Affiliations
Contributions
Ya-lin Peng: Writing—original draft, Investigation, Formal analysis, Data curation. Yong-zhan Wang: Writing—review and editing, Writing—original draft, Validation, Project administration. Yu-cong Li: Supervision, Methodology, Investigation. Li-ting Zeng: Writing—review and editing, Visualization, Data curation. Xin-yi Song: Conceptualization, Validation, Supervision. Xue-qing Li: Data curation, Investigation, Conceptualization. Ao-jing Lv: Validation, Supervision, Methodology. Rui-ling Dong: Validation, Software, Data curation. Wen-hao Gao: Validation, Investigation. Lu Feng: Validation, Conceptualization. He-shuai Li: Supervision, Investigation. Jun Qi: Writing—review and editing, Validation, Supervision, Project administration, Methodology, Investigation, Funding acquisition, Conceptualization. Guo-yuan Xiong: Validation, Supervision, Project administration, Conceptualization. Chun-hui Zhang: Writing—review and editing, Validation, Supervision, Project administration, Conceptualization.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.
About this article
Cite this article
Peng, Yl., Wang, Yz., Li, Yc. et al. A gelatin-stabilized nanosilver particle for visual freshness monitoring of braised chicken meat. npj Sci Food (2026). https://doi.org/10.1038/s41538-026-00816-5
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41538-026-00816-5