Abstract
Heat stress (HS) compromises meat quality through species-specific physiological and epigenetic mechanisms during the growth period. This review investigates how antemortem disruption in energy metabolism, oxidative stress, and proteolysis caused by HS drives diverse postmortem outcomes in poultry, ruminants, and swine. Therefore, these biochemical shifts induced by HS affect consumer preferences for meat, and the direction of change varies across livestock species.
Similar content being viewed by others
Data availability
This review does not include original datasets. All information supporting the analyses and interpretations is contained within the article.
References
Andrenelli, A. et al. International Trade in the Wake of Multiple Shocks: OECD Global Trade Monitor. OECD Trade Policy Papers No. 277 (OECD Publishing, 2023).
Thornton, P., Nelson, G., Mayberry, D. & Herrero, M. Impacts of heat stress on global cattle production during the 21st century: a modelling study. Lancet Planet. Health 6, e192–e201 (2022).
Seneviratne, S. I. et al. Weather and climate extreme events in a changing climate. In Climate Change 2021: The Physical Science Basis. Contribution of Working Group I to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change 1513–1766 (eds Masson-Delmotte, V. et al.) (Cambridge Univ. Press, Cambridge, UK, 2021).
Yates, D. T., White, M. R., Curry, S. A., Hahn, A. A. & White, M. R. The essential (and targetable) role of inflammation in the poor muscle growth and metabolism of heat-stressed livestock. Meat Muscle Biol. 9, 20179 (2025).
Cydne’E, J., Johnson, L. G., Prusa, K. J., Lonergan, S. M. & Huff-Lonergan, E. J. Fresh pork loin pH influences meat quality and the presence of desmin degradation products. Meat Muscle Biol. 9, 18426 (2025).
Wang, Y., Bush, J. & Bohrer, B. M. Meat quality attributes and sensory characteristics for pork tenderloins (M. Psoas Major). Meat Muscle Biol. 9, 20244 (2025).
St-Pierre, N. R., Cobanov, B. & Schnitkey, G. Economic losses from heat stress by US livestock industries. J. Dairy Sci. 86, E52–E77 (2003).
Mia, N., Rahman, M. & Hashem, M. Effect of heat stress on meat quality: a review. Meat Res. 3, 1–8 (2023).
Zhang, M. et al. Impacts of heat stress on meat quality and strategies for amelioration: a review. Int. J. Biometeorol. 64, 1613–1628 (2020).
Von Borell, E. The biology of stress and its application to livestock housing and transportation assessment. J. Anim. Sci. 79, E260–E267 (2001).
Chen, S. et al. Exposure to heat-stress environment affects the physiology, circulation levels of cytokines, and microbiome in dairy cows. Sci. Rep. 8, 14606 (2018).
He, X. et al. Chronic heat stress alters hypothalamus integrity, the serum indexes and attenuates expressions of hypothalamic appetite genes in broilers. J. Therm. Biol. 81, 110–117 (2019).
Huau, G., Liaubet, L., Gourdine, J.-L., Riquet, J. & Renaudeau, D. Multi-tissue metabolic and transcriptomic responses to a short-term heat stress in swine. BMC Genom. 25, 99 (2024).
Hahn, G., Parkhurst, A. & Gaughan, J. Cattle respiration rate as a function of ambient temperature. ASAE Pap. NMC 97, 121 (1997).
Spiers, D. E. Physiological basics of temperature regulation in domestic animals. Environmental Physiology of Livestock (eds Collier, R. J. & Collier, J. L.) 17–34 (Wiley, 2012).
Schneider, P., Beede, D., Wilcox, C. & Collier, R. Influence of dietary sodium and potassium bicarbonate and total potassium on heat-stressed lactating dairy cows. J. Dairy Sci. 67, 2546–2553 (1984).
Baile, C. A. & Forbes, J. M. Control of feed intake and regulation of energy balance in ruminants. Physiol. Rev. 54, 160–214 (1974).
Nonaka, I. et al. Effects of high environmental temperatures on physiological and nutritional status of prepubertal Holstein heifers. Livest. Sci. 113, 14–23 (2008).
Chang-Fung-Martel, J. et al. Negative relationship between dry matter intake and the temperature-humidity index with increasing heat stress in cattle: a global meta-analysis. Int. J. Biometeorol. 65, 2099–2109 (2021).
Kim, H. R. et al. Effects of heat stress on growth performance, physiological responses, and carcass traits in broilers. J. Therm. Biol. 127, 103994 (2025).
Fausnacht, D. W. et al. Heat stress reduces metabolic rate while increasing respiratory exchange ratio in growing pigs. Animals 11, 215 (2021).
de Souza, L. F. A. et al. How heat stress (continuous or cyclical) interferes with nutrient digestibility, energy and nitrogen balances and performance in broilers. Livest. Sci. 192, 39–43 (2016).
Li, X., Zhang, M., Feng, J. & Zhou, Y. Myostatin and related factors are involved in skeletal muscle protein breakdown in growing broilers exposed to constant heat stress. Animals 11, 1467 (2021).
Azad, M., Kikusato, M., Sudo, S., Amo, T. & Toyomizu, M. Time course of ROS production in skeletal muscle mitochondria from chronic heat-exposed broiler chicken. Comp. Biochem. Physiol. Part A Mol. Integr. Physiol. 157, 266–271 (2010).
de Oliveira, M. et al. Effect of constant and cyclic heat stress on growth performance, water intake, and physiological responses in pigs: a meta-analysis. Anim. Feed Sci. Technol. 309, 115904 (2024).
He, S. et al. Impact of heat stress and nutritional interventions on poultry production. Worlds. Poult. Sci. J. 74, 647–664 (2018).
Liu, L., Ren, M., Ren, K., Jin, Y. & Yan, M. Heat stress impacts on broiler performance: a systematic review and meta-analysis. Poult. Sci. 99, 6205 (2020).
Moore, L. D., Le, T. & Fan, G. DNA methylation and its basic function. Neuropsychopharmacology 38, 23–38 (2013).
Hao, Y., Cui, Y. & Gu, X. Genome-wide DNA methylation profiles changes associated with constant heat stress in pigs as measured by bisulfite sequencing. Sci. Rep. 6, 27507 (2016).
Shi, D.-L. & Grifone, R. RNA-binding proteins in the post-transcriptional control of skeletal muscle development, regeneration and disease. Front. Cell Dev. Biol. 9, 738978 (2021).
Page-McCaw, P. S. et al. Collagen IV of basement membranes: I. Origin and diversification of COL4 genes enabling metazoan multicellularity, evolution, and adaptation. J. Biol. Chem. 301, 108496 (2025).
Sugden, M. C. & Holness, M. J. Interactive regulation of the pyruvate dehydrogenase complex and the carnitine palmitoyltransferase system. FASEB J. 8, 54–61 (1994).
Protasi, F. et al. RYR1 and RYR3 have different roles in the assembly of calcium release units of skeletal muscle. Biophys. J. 79, 2494–2508 (2000).
Ren, Z. et al. PNU282987 inhibits amyloid‑β aggregation by upregulating astrocytic endogenous αB‑crystallin and HSP‑70 via regulation of the α7AChR, PI3K/Akt/HSF‑1 signaling axis. Mol. Med. Rep. 22, 201–208 (2020).
Yang, Y. et al. Molecular regulation of whole genome DNA methylation in heat stress response of dairy cows. BMC Genom. 26, 464 (2025).
Vu, M. T. et al. The DNLZ/HEP zinc-binding subdomain is critical for regulation of the mitochondrial chaperone HSPA9. Protein Sci. 21, 258–267 (2012).
Okano, M., Bell, D. W., Haber, D. A. & Li, E. DNA Methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99, 247–257 (1999).
Yossifoff, M., Kisliouk, T. & Meiri, N. Dynamic changes in DNA methylation during thermal control establishment affect CREB binding to the brain-derived neurotrophic factor promoter. Eur. J. Neurosci. 28, 2267–2277 (2008).
Sajjanar, B. et al. Cross-talk between energy metabolism and epigenetics during temperature stress response in C2C12 myoblasts. Int. J. Hyperth. 36, 775–783 (2019).
Guo, X. et al. The expression levels of DNMT3a/3b and their relationship with meat quality in beef cattle. Mol. Biol. Rep. 39, 5473–5479 (2012).
Habashy, W. S., Milfort, M. C., Rekaya, R. & Aggrey, S. E. Molecular and cellular responses of DNA methylation and thioredoxin system to heat stress in meat-type chickens. Animals 11, 1957 (2021).
Peterson, C. L. & Laniel, M.-A. Histones and histone modifications. Curr. Biol. 14, R546–R551 (2004).
Xu, J., Li, C. & Kang, X. The epigenetic regulatory effect of histone acetylation and deacetylation on skeletal muscle metabolism-a review. Front. Physiol. 14, 1267456 (2023).
Lennartsson, A. & Ekwall, K. Histone modification patterns and epigenetic codes. Biochim. Biophys. Acta Gen. Subj. 1790, 863–868 (2009).
David, S. -A. et al. Thermal manipulation during embryogenesis impacts H3K4me3 and H3K27me3 histone marks in chicken hypothalamus. Front. Genet. 10, 1207 (2019).
Liu, Z. et al. Chronic heat stress inhibits glycogen synthesis through gga-miR-212-5p/GYS1 axis in the breast muscle of broilers. Poult. Sci. 103, 103455 (2024).
Sengar, G. S. et al. Identification of differentially expressed microRNAs in Sahiwal (Bos indicus) breed of cattle during thermal stress. Cell Stress Chaperones 23, 1019–1032 (2018).
Yadav, P. et al. Differential expression of miRNAs and related mRNAs during heat stress in buffalo heifers. J. Therm. Biol. 97, 102904 (2021).
González-Blanco, L. et al. Exploring the miRNAs profile in dark-cutting beef. Foods 13, 960 (2024).
Guttman, M. & Rinn, J. L. Modular regulatory principles of large non-coding RNAs. Nature 482, 339–346 (2012).
Salmena, L., Poliseno, L., Tay, Y., Kats, L. & Pandolfi, P. P. A ceRNA hypothesis: the Rosetta Stone of a hidden RNA language? cell 146, 353–358 (2011).
Liu, Z. et al. Unraveling the role of long non-coding RNAs in chronic heat stress-induced muscle injury in broilers. J. Anim. Sci. Biotechnol. 15, 135 (2024).
Zhang, Z. Y. et al. Effects of constant and cyclic heat stress on muscle metabolism and meat quality of broiler breast fillet and thigh meat. Poult. Sci. 91, 2931–2937 (2012).
Lu, Z. et al. Chronic heat stress impairs the quality of breast-muscle meat in broilers by affecting redox status and energy-substance metabolism. J. Agric. Food Chem. 65, 11251–11258 (2017).
Xing, T., Xu, X., Zhou, G., Wang, P. & Jiang, N. The effect of transportation of broilers during summer on the expression of heat shock protein 70, postmortem metabolism and meat quality. J. Anim. Sci. 93, 62–70 (2015).
Yang, P. et al. The expression of carnosine and its effect on the antioxidant capacity of longissimus dorsi muscle in finishing pigs exposed to constant heat stress. Asian-Australas. J. Anim. Sci. 27, 1763 (2014).
Zhang, M. et al. An early-postmortem metabolic comparison among three extreme acute heat stress temperature settings in chicken breast muscle. J. Food Sci. Technol. 58, 4823–4829 (2021).
Chen, S. et al. Dietary rutin improves breast meat quality in heat-stressed broilers and protects mitochondria from oxidative attack via the AMPK/PINK1–PARKIN pathway. J. Sci. Food Agric. 103, 2367–2377 (2023).
Guo, Z. et al. Dihydromyricetin improves meat quality and promotes skeletal muscle fiber type transformations via AMPK signaling in growing–finishing pigs. Food Funct. 13, 3649–3659 (2022).
Wang, R. H. et al. Effect of acute heat stress and slaughter processing on poultry meat quality and postmortem carbohydrate metabolism. Poult. Sci. 96, 738–746 (2017).
Lebret, B., Serviento, A. M. & Renaudeau, D. Pork quality traits and associated muscle metabolic changes in pigs under chronic prenatal and postnatal heat stress. J. Anim. Sci. 101, skad305 (2023).
Liu, Y. et al. Hydroxy selenomethionine improves meat quality through optimal skeletal metabolism and functions of selenoproteins of pigs under chronic heat stress. Antioxidants 10, 1558 (2021).
Shi, Z. et al. Effects of high ambient temperature on meat quality, serum hormone concentrations, and gene expression in the longissimus dorsi muscle of finishing pigs. Anim. Prod. Sci. 57, 1031–1039 (2016).
Feng, J., Zhang, M., Zheng, S., Xie, P. & Ma, A. Effects of high temperature on multiple parameters of broilers in vitro and in vivo. Poult. Sci. 87, 2133–2139 (2008).
Aryal, B. et al. Major oxidative and antioxidant mechanisms during heat stress-induced oxidative stress in chickens. Antioxidants 14, 471 (2025).
Xing, T. et al. Different oxidative status and expression of calcium channel components in stress-induced dysfunctional chicken muscle. J. Anim. Sci. 95, 1565–1573 (2017).
Wang, R. R., Pan, X. J. & Peng, Z. Q. Effects of heat exposure on muscle oxidation and protein functionalities of pectoralis majors in broilers. Poult. Sci. 88, 1078–1084 (2009).
Segura, J. et al. Alleviating heat stress in fattening pigs: low-intensity showers in critical hours alter body external temperature, feeding pattern, carcass composition, and meat quality characteristics. Animals 14, 1661 (2024).
Chen, Z. et al. Oxidative stress impairs the meat quality of broiler by damaging mitochondrial function, affecting calcium metabolism and leading to ferroptosis. Anim. Biosci. 35, 1616 (2022).
Kadim, I., Mahgoub, O. & Khalaf, S. Effects of the transportation during hot season and electrical stimulation on meat quality characteristics of goat Longissimus dorsi muscle. Small Rumin. Res. 121, 120–124 (2014).
Wang, L.-L., Han, L., Ma, X.-L., Yu, Q.-L. & Zhao, S.-N. Effect of mitochondrial apoptotic activation through the mitochondrial membrane permeability transition pore on yak meat tenderness during postmortem aging. Food Chem. 234, 323–331 (2017).
Xing, T. et al. Expression of heat shock protein 70 in transport-stressed broiler pectoralis major muscle and its relationship with meat quality. Animal 11, 1599–1607 (2017).
Li, C.-Y., Lee, J.-S., Ko, Y.-G., Kim, J.-I. & Seo, J.-S. Heat shock protein 70 inhibits apoptosis downstream of cytochrome c release and upstream of caspase-3 activation. J. Biol. Chem. 275, 25665–25671 (2000).
Ma, Y. et al. New perspective for calpain-mediated regulation of meat quality: unveiling the impact on mitochondrial pathway apoptosis in post-mortem. Food Chem. 441, 138287 (2024).
Huang, F. et al. Cleavage of the calpain inhibitor, calpastatin, during postmortem ageing of beef skeletal muscle. Food Chem. 148, 1–6 (2014).
Barnoy, S. & Kosower, N. S. Caspase-1-induced calpastatin degradation in myoblast differentiation and fusion: cross-talk between the caspase and calpain systems. FEBS Lett. 546, 213–217 (2003).
Devapriya, A. et al. Analysis of carcass traits and quantitative expression patterns of different meat quality governing genes during heat stress exposure in indigenous goats. Food Chem. Mol. Sci. 3, 100052 (2021).
Roths, M. et al. Effects of heat stress on markers of skeletal muscle proteolysis in dairy cattle. J. Dairy Sci. 106, 5825–5834 (2023).
Zaboli, G., Huang, X., Feng, X. & Ahn, D. U. How can heat stress affect chicken meat quality?–a review. Poult. Sci. 98, 1551–1556 (2019).
Goll, D. E., Thompson, V. F., Li, H., Wei, W. & Cong, J. The calpain system. Physiol. Rev. 83, 731–801 (2003).
Akşit, M., Yalcin, S., Özkan, S., Metin, K. & Özdemir, D. Effects of temperature during rearing and crating on stress parameters and meat quality of broilers. Poult. Sci. 85, 1867–1874 (2006).
Lu, Q., Wen, J. & Zhang, H. Effect of chronic heat exposure on fat deposition and meat quality in two genetic types of chicken. Poult. Sci. 86, 1059–1064 (2007).
Wang, R., Pan, X. & Peng, Z. Effects of heat exposure on muscle oxidation and protein functionalities of pectoralis majors in broilers. Poult. Sci. 88, 1078–1084 (2009).
Dai, S. F. et al. Effects of dietary glutamine and gamma-aminobutyric acid on meat colour, pH, composition, and water-holding characteristic in broilers under cyclic heat stress. Br. Poult. Sci. 53, 471–481 (2012).
Macías-Cruz, U. et al. Feedlot growth, carcass characteristics and meat quality of hair breed male lambs exposed to seasonal heat stress (winter vs. summer) in an arid climate. Meat Sci. 169, 108202 (2020).
Kim, Y. H. B. et al. Understanding postmortem biochemical processes and post-harvest aging factors to develop novel smart-aging strategies. Meat Sci. 144, 74–90 (2018).
Kadim, I. et al. The influence of season on quality characteristics of hot-boned beef m. longissimus thoracis. Meat Sci. 66, 831–836 (2004).
Kadim, I. et al. The influence of seasonal temperatures on meat quality characteristics of hot-boned, m. psoas major and minor, from goats and sheep. Meat Sci. 80, 210–215 (2008).
Kadim, I. T. et al. Effects of transportation during the hot season, breed and electrical stimulation on histochemical and meat quality characteristics of goat longissimus muscle. Anim. Sci. J. 81, 352–361 (2010).
Kadim, I. et al. Effect of transportation at high ambient temperatures on physiological responses, carcass and meat quality characteristics in two age groups of Omani sheep. Asian-Australas. J. Anim. Sci. 20, 424–431 (2007).
Pardo, Z. et al. Impact of heat stress on meat quality and antioxidant markers in Iberian pigs. Antioxidants 10, 1911 (2021).
Tuell, J. R., Nondorf, M. J., Maskal, J. M., Johnson, J. S. & Kim, Y. H. B. Impacts of in utero heat stress on carcass and meat quality traits of market weight gilts. Animals 11, 717 (2021).
Morgado, J. N. et al. Effects of management strategies on animal welfare and productivity under heat stress: a synthesis. Front. Vet. Sci. 10, 1145610 (2023).
Chauhan, S. S., Rashamol, V., Bagath, M., Sejian, V. & Dunshea, F. R. Impacts of heat stress on immune responses and oxidative stress in farm animals and nutritional strategies for amelioration. Int. J. Biometeorol. 65, 1231–1244 (2021).
Herrera, N. J. et al. Beef color stability and composition in cattle fed high levels of vitamin E following prolonged aging. Meat Muscle Biol. 8, 18008 (2024).
Kelley, M. et al. Impact of feeding different fat sources and levels of vitamin E isoforms to heavy slaughter weight (150 kg) Pigs: I. Carcass characteristics and fresh pork quality. Meat Muscle Biol. 9, 19043 (2025).
Kim, B.-M. et al. Role of vitamin E on bovine skeletal-muscle-derived cells from Korean native cattle under heat treatment. J. Anim. Sci. 102, skae292 (2024).
Nawaz, A. H. et al. Poultry response to heat stress: Its physiological, metabolic, and genetic implications on meat production and quality including strategies to improve broiler production in a warming world. Front. Vet. Sci. 8, 699081 (2021).
Chen, Y., Cheng, Y., Du, M. & Zhou, Y. Protective effects of dietary synbiotic supplementation on meat quality and oxidative status in broilers under heat stress. Environ. Sci. Pollut. Res. 28, 30197–30206 (2021).
Kim, H. W., Kim, J. H., Yan, F., Cheng, H. W. & Brad Kim, Y. H. Effects of heat stress and probiotic supplementation on protein functionality and oxidative stability of ground chicken leg meat during display storage. J. Sci. Food Agric. 97, 5343–5351 (2017).
El-Tahan, H. M. et al. Fish oil a source of omega-3 fatty acids affects hypothalamus heat resistance genes expressions and fatty acid composition in heat-stressed chicks. Domest. Anim. Endocrinol. 91, 106915 (2025).
Elsherbeni, A. I. et al. The role of trimethylglycine (betaine) as an anti-heat stress agent in sustainable poultry production: enhancing growth, stress resilience, and nutrient utilization. J. Therm. Biol. 130, 104152 (2025).
Jimoh, O. A., Daramola, O. T., Okin-Aminu, H. O. & Ojo, O. A. Performance, hemato-biochemical indices and oxidative stress markers of broiler chicken fed phytogenic during heat stress condition. J. Anim. Sci. Technol. 64, 970 (2022).
Jimoh, O. A., Daramola, O. T., Okin-Aminu, H. O., Ojo, O. A. & Oyeyemi, W. A. Effect of phytogenic supplements on the reproductive physiology and metabolic hormones of rabbits exposed to heat stress conditions. J. Therm. Biol. 112, 103438 (2023).
Sahin, K., Orhan, C., Tuzcu, Z., Tuzcu, M. & Sahin, N. Curcumin ameloriates heat stress via inhibition of oxidative stress and modulation of Nrf2/HO-1 pathway in quail. Food Chem. Toxicol. 50, 4035–4041 (2012).
Zhang, J. et al. Curcumin attenuates heat-stress-induced oxidant damage by simultaneous activation of GSH-related antioxidant enzymes and Nrf2-mediated phase II detoxifying enzyme systems in broiler chickens. Poult. Sci. 97, 1209–1219 (2018).
Sahin, K. et al. Resveratrol protects quail hepatocytes against heat stress: modulation of the Nrf2 transcription factor and heat shock proteins. J. Anim. Physiol. Anim. Nutr. 96, 66–74 (2012).
Liang, Q. et al. Screening of heat stress-related biomarkers in chicken serum through label-free quantitative proteomics. Poult. Sci. 103, 103340 (2024).
Tang, S., Yu, J., Zhang, M. & Bao, E. Effects of different heat stress periods on various blood and meat quality parameters in young Arbor Acer broiler chickens. Can. J. Anim. Sci. 93, 453–460 (2013).
Debut, M. et al. Variation of chicken technological meat quality in relation to genotype and preslaughter stress conditions. Poult. Sci. 82, 1829–1838 (2003).
Holm, C. P. & Fletcher, D. L. Antemortem holding temperatures and broiler breast meat quality. J. Appl. Poult. Res. 6, 180–184 (1997).
Sandercock, D., Hunter, R. R., Nute, G. R., Mitchell, M. & Hocking, P. M. Acute heat stress-induced alterations in blood acid-base status and skeletal muscle membrane integrity in broiler chickens at two ages: Implications for meat quality. Poult. Sci. 80, 418–425 (2001).
Dai, S., Wang, L., Wen, A., Wang, L. & Jin, G. Dietary glutamine supplementation improves growth performance, meat quality and colour stability of broilers under heat stress. Br. Poult. Sci. 50, 333–340 (2009).
Zhang, M. et al. Effect of different short-term high ambient temperature on chicken meat quality and ultra-structure. Asian-Australas. J. Anim. Sci. 32, 701–710 (2019).
Awad, E. A., Najaa, M., Zulaikha, Z. A., Zulkifli, I. & Soleimani, A. F. Effects of heat stress on growth performance, selected physiological and immunological parameters, caecal microflora, and meat quality in two broiler strains. Asian-Australas. J. Anim. Sci. 33, 778–787 (2020).
Zeferino, C. P. et al. Carcass and meat quality traits of chickens fed diets concurrently supplemented with vitamins C and E under constant heat stress. Animal 10, 163–171 (2016).
Cartoni Mancinelli, A. et al. Impact of chronic heat stress on behavior, oxidative status and meat quality traits of fast-growing broiler chickens. Front. Physiol. 14, 1242094 (2023).
Imik, H. et al. Meat quality of heat stress exposed broilers and effect of protein and vitamin E. Br. Poult. Sci. 53, 689–698 (2012).
Xing, T., Xu, X. L., Zhou, G. H., Wang, P. & Jiang, N. N. The effect of transportation of broilers during summer on the expression of heat shock protein 70, postmortem metabolism and meat quality. J. Anim. Sci. 93, 62–70 (2015).
Li, Z. et al. Investigation into the effects of acute heat stress on stress level, meat quality, myofibrillar proteins properties and serum metabolites release of Muscovy ducks (Cairina moschata). Food Chem. 482, 144104 (2025).
Froning, G., Babji, A. & Mather, F. The effect of preslaughter temperature, stress, struggle and anesthetization on color and textural characteristics of turkey muscle. Poult. Sci. 57, 630–633 (1978).
McKee, S. R. & Sams, A. R. The effect of seasonal heat stress on rigor development and the incidence of pale, exudative turkey meat. Poult. Sci. 76, 1616–1620 (1997).
Owens, C. M., McKee, S. R., Matthews, N. S. & Sams, A. R. The development of pale, exudative meat in two genetic lines of turkeys subjected to heat stress and its prediction by halothane screening. Poult. Sci. 79, 430–435 (2000).
Kang, S. M., Lee, I. S. & Lee, S. K. The quality characteristics of M. longissimus from Hanwoo (Korean cattle) steer with different raising altitudes and slaughter seasons. Livest. Sci. 136, 240–246 (2011).
Węglarz, A. Meat quality defined based on pH and colour depending on cattle category and slaughter season. Czech J. Anim. Sci. 55, 548–556 (2010).
Mitlohner, F., Galyean, M. & McGlone, J. Shade effects on performance, carcass traits, physiology, and behavior of heat-stressed feedlot heifers. J. Anim. Sci. 80, 2043–2050 (2002).
Kang, S. M., Lee, I. S. & Lee, S. K. Carcass characteristics of Hanwoo (Korean cattle) from different sex conditions, raising altitudes and slaughter seasons. Livest. Sci. 123, 283–287 (2009).
Zhang, M. et al. Impact of heat stress on the growth performance and retail meat quality of 2nd cross (Poll Dorset×(Border Leicester× Merino)) and Dorper lambs. Meat Sci. 181, 108581 (2021).
Liu, H., Li, K., Mingbin, L., Zhao, J. & Xiong, B. Effects of chestnut tannins on the meat quality, welfare, and antioxidant status of heat-stressed lambs. Meat Sci. 116, 236–242 (2016).
Subhopoto, M. et al. Effects of grazing duration on growth, carcass characteristics, and meat quality of Kachhi sheep under heat stress conditions. Acta Vet. 66, 172–186 (2025).
Hashem, M., Hossain, M., Rana, M., Islam, M. & Saha, N. Effect of heat stress on blood parameter, carcass and meat quality of Black Bengal goat. Bangladesh J. Anim. Sci. 42, 57–61 (2013).
Čobanović, N. et al. The influence of pre-mortem conditions on pale, soft and exudative (PSE) and dark, firm and dry (DFD) pork meat. Acta Veterinaria-Beograd. 66, 172–186 (2016).
Cruzen, S. et al. Carcass composition of market weight pigs subjected to heat stress in utero and during finishing. J. Anim. Sci. 93, 2587–2596 (2015).
Serviento, A. M., Lebret, B. & Renaudeau, D. Chronic prenatal heat stress alters growth, carcass composition, and physiological response of growing pigs subjected to postnatal heat stress. J. Anim. Sci. 98, skaa161 (2020).
Acknowledgements
This work was supported by the Brain Pool program funded by the Ministry of Science and Information and Communication Technology through the National Research Foundation of Korea (grant number:2022H1D3A2A01096260). In part, this work was also supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (RS-2025-00559561).
Author information
Authors and Affiliations
Contributions
J.H.L.: conceptualization, visualization, writing-original draft; X.C.J.: writing-review, and editing. W.S.K.: writing-review, and editing. H.G.L.: funding acquisition, writing-review, and editing. Y.H.B.K.: funding acquisition, project administration, supervision, writing-review, and editing.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.
About this article
Cite this article
Lee, J.H., Jin, X.C., Kim, W.S. et al. Unraveling the impact of heat stress on meat quality: integrating physiology, epigenetics, and postmortem biochemistry. npj Sci Food (2026). https://doi.org/10.1038/s41538-026-00840-5
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41538-026-00840-5


