Fig. 2: Auditory input targets the lateral amygdala (LA).
From: Diversity of interneurons in the lateral and basal amygdala
a, b Virus encoding ChR2-eYFP was stereotactically targeted to the auditory thalamus (AT) a or the auditory cortex (AC) b. ChR2-eYFP expressing afferents from the AT (c) and AC (d) were detected throughout the rostro-caudal extent of the BLA (right). Schematics (left) show corresponding locations of interneurons that that received AT input (green), AC input (blue) or no input (red). e, f Shown are representative cells in the LA (e) and BA (f) recovered following immunohistochemistry for biocytin. AT afferents (CHr2-eYFP) are also shown (green) highlighting the higher density of auditory projections within the LA (e) compared to the BA (f). Insets show respective somatic GAD67-eGFP fluorescence for GABAergic identification. g Within-slice ratio of BA:LA fluorescence across the rostro-caudal range (rostral BLA, AT = 0.42 ± 0.1 (n = 7), AC = 0.47 ± 0.07 (n = 4), p = 0.6695; middle BLA, AT = 0.37 ± 0.07 (n = 10), AC = 0.48 ± 0.08 (n = 9), p = 0.3219; caudal BLA, AT = 0.34 ± 0.05 (n = 6), AC = 0.43 ± 0.7 (n = 9), p = 0.3403). Mean ± SEM (unpaired two-tailed t test with Welch’s correction).
