Fig. 4

a Quantitative real-time PCR of mitotic CLB transcripts in yeast cells treated with nocodazole. Total mRNA was isolated from arrested wild type, fkh2Δ, clb3Δclb4Δ, or clb5Δclb6Δ cells, and CLB2 and CLB3 mRNA levels were measured. ACT1 and TSA1 genes were used as negative controls, as they are not affected by cell cycle dynamics. Error bars on the histograms represent SDs from the mean of three independent experiments; p-values are indicated on the histograms in Supplementary Fig. S5h. Nocodazole-arrested cells show that clb3Δclb4Δ affects CLB2 transcript accumulation, whereas clb5Δclb6Δ affects both CLB2 and CLB3 transcript accumulation. b Schematic view of the regulatory connections involving the Fkh/Ndd1 and Clb/Cdk1 complexes. Colored solid arrows refer to the known interactions reported in literature; colored dashed arrows refer to the newly unraveled interactions between Fkh2/Ndd1 and Clb/Cdk1 complexes; gray dashed arrows refer to the newly unraveled interactions between Fkh1/Ndd1 and Clb/Cdk1 complexes. See text for details