Fig. 3
From: Next-generation mammalian genetics toward organism-level systems biology
One-step generation of genome-edited mice. a An example of triple-CRISPR KO littermates (before weaning) in B6 strain. Tyr gene coding tyrosinase (an enzyme involving black coat color) was knocked out by the triple-CRISPR method.62 All littermates had white coat color, indicating ~100% bi-allelic KO rate of the targeted gene. b An example of B6 ES mouse littermates (before weaning) by 3i + LIF culture and 8-cell injection.141 All littermates had black coat color, indicating efficient generation of ~100% ESC-derived mouse. c An ES mouse embryo (E13.5) derived from an H2B-EGFP KI ESC clone.170 Only the embryo (but not the extraembryonic tissues) expresses EGFP, suggesting the unique contribution of ESC-derived cells. Entire section of the ES mouse is shown in the right panel. All animal experiments here were approved by the Institutional Animal Care and Use Committee of RIKEN Kobe Branch, and all of the animal care was in accordance with the Institutional Guidelines
