Fig. 2
Images and quantitative analysis of single-cell transcription for SIC1, CLN2, and CLB5 under optimal growth conditions in an asynchronous cell population. a Detection at single molecule resolution of endogenous SIC1, CLN2, and CLB5 transcriptional expressions with smFISH (red dots: mRNA molecules; blue: DAPI-stained nucleus). White arrows point towards transcription sites. Scale bar represents 5 μm. b Experimental and simulated distributions of mRNA number per cell for SIC1, CLN2, and CLB5 for the entire population (n > 900 cells). The overall averages are 4.53, 6.46, and 1.93 and medians are 2, 1, and 1 mRNAs/cell for SIC1, CLN2, and CLB5, respectively. c Measured and simulated mRNA distributions for each cell cycle phase. Lower and upper quartiles form boundaries of the boxes. Lines and white dots insight the boxes represent medians and mean values, respectively. Whiskers are given by 1.5 × IQR (interquartile range). Data points outside of the IQR define outliers. For better overview early G1 and late G1 are abbreviated to eG1 and lG1, respectively. In b and c experimental and simulated data are represented by colored and gray bars or boxes, respectively. d Fraction of cells with transcription sites (TS) in each cell cycle phase. Lines between data points are for visualization only. e mRNA distributions for each cell cycle phase grouped as follows: 0, 1–5, 6–11, 12–20, 21–30, >30 number of mRNA per cell (n > 900 cells see Supplementary Table S1 for data)
