Fig. 3: Event map and event sparkline showing multiomics events during epiblast differentiation.

Shows 64 multiomics events inferred from 40 clusters: 4 clusters comprise 3564 phosphosite profiles (red), 24 clusters comprise 6225 mRNA profiles (purple), and 12 clusters comprise 2735 protein profiles (green; see Supplementary Figs 4–6). a Event map of key phosphorylation, transcription, and translation events, indicated with up and down arrows. Each horizontal bar represents one cluster, and indicates when it has increased abundance. Each column (gray or white shading) indicates a group of events that occur at significantly different times to all other events. Events with the same horizontal positioning are assessed to occur simultaneously. Dotted gray lines indicate overlapping but non-simultaneous events. b Event sparkline summarizing the inferred temporal order of events shown in above event map. Events are labeled with cluster numbers. Gray lines or arcs indicate overlapping but non-simultaneous events. The large group of 22 overlapping events (“Phase 2”) drive exit from the naive state; prior events (“Phase 1”) mostly relate to stem cell renewal; later events (“Phase 3”) drive epiblast induction (see Supplementary note). Figures made using data from Yang et al.⁸ with Minardo-Model and edited in Adobe Illustrator.