Fig. 3: Investigation of the essential nutritional requirements of BB-12 and BB-46. | npj Systems Biology and Applications

Fig. 3: Investigation of the essential nutritional requirements of BB-12 and BB-46.

From: Identifying the essential nutritional requirements of the probiotic bacteria Bifidobacterium animalis and Bifidobacterium longum through genome-scale modeling

Fig. 3: Investigation of the essential nutritional requirements of BB-12 and BB-46.

a Potentially essential nutrients for growth of BB-12 and BB-46 that are missing in the generic culture medium, as identified through in silico analysis and literature. The requirement for different nutrients was evaluated in vitro through nutrient omission experiments. b Heat map comparing the in silico predictions and the in vitro results regarding vitamin and amino acid requirements of BB-12 and BB-46. Blue: not essential, red: essential, gray: not tested. Knowledge gained from the validation experiments was incorporated into iAZ480 and iMS520 through the addition of new reactions or deletion of existing reactions so that the model predictions agree with the in vitro results.

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