Table 2 Comparison of in vitro and in silico reaction rates for cultivation of BB-12 and BB-46 in the newly formulated chemically defined medium.

Growth rate [h⁻¹]

0.45 ± 0.0

0.44

0.35 ± 0.0

0.34

Sucrose uptake rate [mmol g_CDW⁻¹ h⁻¹]

3.9 ± 0.2

3.9

2.8 ± 0.4

2.8

Acetate:lactate ratio [-]

2.5 ± 0.0

2.5

17.3 ± 0.0

17.3

Acetate secretion rate [mmol g_CDW⁻¹ h⁻¹]

11.8 ± 0.7

12.0

11.9 ± 0.3

10.4

Lactate secretion rate [mmol g_CDW⁻¹ h⁻¹]

4.7 ± 0.3

4.8

0.7 ± 0.0

0.6

Formate secretion rate [mmol g_CDW⁻¹ h⁻¹]

0.3 ± 0.0

3.3

4.0 ± 0.2

5.8

Succinate secretion rate [mmol g_CDW⁻¹ h⁻¹]

0.2 ± 0.0

0.2

0.2 ± 0.0

0.1

Ethanol secretion rate [mmol g_CDW⁻¹ h⁻¹]

–

0.4

2.5 ± 0.3

1.7

Carbon recovery excluding biomass formation [%]

82

–

106

–