Fig. 9: Beeswarm plots in log10 scale of DNA-seq and RNA repertoires. | npj Systems Biology and Applications

Fig. 9: Beeswarm plots in log10 scale of DNA-seq and RNA repertoires.

From: Understanding repertoire sequencing data through a multiscale computational model of the germinal center

Fig. 9

Beeswarm plots in log10 scale of (a) DNA-seq and (b) RNA repertoires at day 21 of the GC reaction generated by a representative simulation. Each dot represents a clone, some of which are a mixture of B cells, MBCs and/or PCs. In both cases, we find 5 and 13 dominant clones using the 75th percentile or 0.5% threshold, respectively. Dot colors indicate the fraction of PC BcR sequences within each clone, whose range is about a factor of 100 times larger for the RNA-based repertoire. The size of the symbol represents the median affinity of that clone (small symbol: affinity below the 75th percentile). The horizontal lines denote the 75th percentile (red) and 0.5% (purple) abundance thresholds to define dominant clones.

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