Fig. 4: Model agreement with the lipolysis data.

Released glycerol (left) was measured using microdialysis in the adipose tissue in situ⁴³. All other data (middle, right) of released glycerol, released fatty acid (FA), phosphorylation of hormone-sensitive lipase (HSL) on Serine 552, and phosphorylation of protein kinase B (PKB) on serine 308 or threonine 473 were measured in isolated human adipocytes²³. In all panels, lines represent the model simulation with the best agreement to data, the shaded areas represent the model uncertainty, and experimental data points are represented as mean values with error bars (SEM). Simulations and experimental data in red correspond to experiments under type 2 diabetic conditions, and in blue under non-diabetic conditions. Dashed lines and experimental data with open triangles were not used to estimate the model parameters. Light/dark gray horizontal bars indicate adrenergic stimulation with epinephrine (epi) or isoprenaline (iso), and black horizontal bars in the left figures indicate insulin (ins) stimulation. The model agreement was assessed with a χ²-test, as described in the “Statistical analysis” section.