Fig. 5: COX-2 activated microglial cells and consequently induces inflammation by activating the PGE2/IL-22 axis. | npj Systems Biology and Applications

Fig. 5: COX-2 activated microglial cells and consequently induces inflammation by activating the PGE2/IL-22 axis.

From: The antidepressive mechanism of Longya Lilium combined with Fluoxetine in mice with depression-like behaviors

Fig. 5

A Western blot analysis of COX-2, PGE2, IL-22, TNF-α, and IFN-γ proteins in LPS-induced cells treated with Longya Lilium + fluoxetine. LPS-induced cells were treated with si-COX-2, si-COX-2 + oe-PGE2, oe-PGE2 or oe-PGE2 + si-IL-22. B COX-2, PGE2, IL-22, TNF-α, and IFN-γ expression in the supernatant of LPS-induced cells measured by ELISA. C Cell viability measured by MTT assay. D Cell apoptosis measured by flow cytometry. Measurement data were expressed as mean ± standard deviation. One-way or two-way ANOVA with Tukey’s post-hoc test was used to compare multiple groups. The cell experiment was repeated three times independently. *p < 0.05, compared with the si-NC-treated cells. #p < 0.05, compared with the si-COX-2-treated cells. &p < 0.05, compared with the oe-NC-treated cells. $p < 0.05, compared with the oe-PGE2-treated cells.

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