Fig. 6: Ab-seq peptides that map to a specific clonotype can be utilized to recover clonal information. | npj Systems Biology and Applications

Fig. 6: Ab-seq peptides that map to a specific clonotype can be utilized to recover clonal information.

From: Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling

Fig. 6: Ab-seq peptides that map to a specific clonotype can be utilized to recover clonal information.

Uniquely mapped CDR3-overlapping peptides (quantified in Fig. 5f) were utilized to recover the clonotype information provided by BCR-seq, with overlapping segment in bold. a Example of Ab-seq peptides mapped to a bulkBCR-seq used to recover the single chain V(D)J sequence. b Example of Ab-seq peptides mapped to a scBCR-seq used to recover the paired chain V(D)J sequence by utilizing the cell barcode unique to each single-cell droplet. See Supplementary File 1 for the full table of all V(D)J sequences recovered by Ab-seq.

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