Table 1 Comparison between GeoMx DSP nanostring and MIST
From: Microarray integrated spatial transcriptomics (MIST) for affordable and robust digital pathology
Comparison parameters | GeoMx DSP Nanostring | MIST (VISIUM) |
|---|---|---|
Setup cost | Requires GeoMx digital spatial profiling instrument ($295,000) | Benchtop (no investment) |
Single slide processing cost | 10,000 USD for one ROI assaying up to 50 AOIs of 200um diameter | 400–500 spots/ROI: ~300–800 USD. 20 such ROIs can be processed per slide *spots(50um diameter are analogous to AOIs) |
Choice of sampling | Should be ideally homogenous for a specific ROI | Size of spots is fixed- 50um Heterogenous sampling is ideal- captures maximum heterogeneity |
ROI analysis e.g. 2 mm ROI | Less comprehensive; AOIs, maximum in tens are assayed | More comprehensive, entire 2 mm area is assayed |
Resolution | Larger AOI- more cells-mini bulk of all the cells in the AOI | Fixed resolution corresponding to the spot size(50um) |
