Fig. 1: PD-MIRO1 mutant organoids show sustained dopaminergic neuron loss and altered astrocyte differentiation. | npj Systems Biology and Applications

Fig. 1: PD-MIRO1 mutant organoids show sustained dopaminergic neuron loss and altered astrocyte differentiation.

From: MIRO1 mutation leads to metabolic maladaptation resulting in Parkinson’s disease-associated dopaminergic neuron loss

Fig. 1

a Representative images of 20 and 30 day old organoids staining for neurons (TUJ1, red), dopaminergic neurons (TH, green) and nuclei (Hoechst, blue). Scale bar = 200 µm. b Quantification of total neuronal population within organoids over time quantified by pixel volume ratio (TUJ1/nuclei). n = 8 to 12 from at least 4 independent organoid derivations. c Quantification of total dopaminergic neuron population within organoids over time quantified by pixel volume ratio ((TH + TUJ1)/TUJ1). d Representative Images of 60 day old astrocytes within WT and PD organoids using GFAP (green), S100B (red) and Hoechst (nuclei, blue). Scale bar = 200 µm. e Quantification of total astrocyte population within organoids over time quantified by pixel volume ratio ((GFAP + S100B)/nuclei). n = 6 to 8 from at least 3 independent derivations. f Expression levels of GFAP protein over time quantified by the mean intense fluorescence. Statistical significance was tested with Kruskall-Walis test.*p < 0.05 (WT vs PD); ##p < 0.01 (PD vs GC); $$$p < 0.001 (WT vs GC). g Expression levels of GFAP protein over time quantified by the mean intense fluorescence. Statistical significance was tested with Kruskall-Walis test.*p < 0.05 (WT vs PD); $p < 0.05 (WT vs GC); **p < 0.01 (WT vs PD); ##p < 0.01 (PD vs GC). b, c, e Statistical significance was tested with the Kruskall-Walis test and represented with ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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