Fig. 1

Translation of pre-F hRSV F stabilization to bRSV F. a Structural model of a pre-F hRSV F trimer stabilized by DS-Cav1 mutations (PDB ID 4MMU).¹⁷ One monomer is depicted by a blue ribbon model with the four DS-Cav1 mutations shown by red stick models outlined by red squares. The other two monomers are depicted by gray surface representations. b Sequence variation between hRSV strain A2 and eight different bRSV strains is mapped (orange surface representation) onto a blue ribbon model of one monomer of a DS-Cav1 pre-F RSV F trimer colored as in a. c The locations of the DS-Cav1 mutations (red), the sc linkage (green) and interprotomer disulfide stabilization mutations (green) introduced into bovine RSV F protein are indicated by boxed stick models for one RSV F monomer. In b and c, the other two monomers of the trimer are shown as gray surface representations. d Phylogenetic tree for human (blue) and bovine (orange) RSV F proteins. Names shown indicate the virus strain or isolate with study usage shown in parentheses. GenBank accession numbers for all strains used in this study are shown in Supplementary Table 1