Fig. 5
From: Genetic stability of Rift Valley fever virus MP-12 vaccine during serial passages in culture cells
Detection of reversion mutants of rMP12-ΔNSs16/198 during passages in Vero cells. Recombinant MP-12 encoding a 69% in-frame truncation of NSs open reading frame (rMP12-ΔNSs16/198) was passaged 25 times in Vero cells at MOI of 0.01 or less at 3 days post infection. The presence of reversion mutations are shown. a M-segment nt. 795: HEX-795-C and FAM-795-T, b M-segment nt. 3564: HEX-3564-G and FAM-3564-A, c L-segment nt. 533: HEX-533-C and FAM-533-T, d L-segment nt. 3104: HEX-3104-A and FAM-3104-G, and e L-segment nt. 3750: HEX-3750-A and FAM-3750-G. Two independent studies are shown (Exp-1 and 2). Relative percentages of RNA copy numbers for parental and reversion mutants are shown in blue and red bars, respectively. The bar labels in red represent the percentages of reversion mutants
